Metal Nanoparticles with Antimicrobial Properties: The Toxicity Response in Mouse Mesenchymal Stem Cells

Rössner ml., Pavel; Červená, Tereza; Echalar, Barbora; Palacká, Kateřina; Milcová, Alena; Novaková, Zuzana; Šíma, Michal; Šímová, Zuzana; Vaňková, Jolana; Holáň, Vladimír

doi:https://dx.doi.org/10.3390/toxics11030253

Number of the records: 1

Metal Nanoparticles with Antimicrobial Properties: The Toxicity Response in Mouse Mesenchymal Stem Cells

1.

SYSNO ASEP	0581955
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Metal Nanoparticles with Antimicrobial Properties: The Toxicity Response in Mouse Mesenchymal Stem Cells
Author(s)	Rössner ml., Pavel (UEM-P)_{RID, ORCID} Červená, Tereza (UEM-P)_{ORCID, RID} Echalar, Barbora (UEM-P) Palacká, Kateřina (UEM-P)_ORCID Milcová, Alena (UEM-P) Novaková, Zuzana (UEM-P) Šíma, Michal (UEM-P)_{RID, ORCID} Šímová, Zuzana (UEM-P)_ORCID Vaňková, Jolana (UEM-P) Holáň, Vladimír (UEM-P)_RID
Article number	253
Source Title	Toxics. - : MDPI Roč. 11, č. 3 (2023)
Number of pages	21 s.
Language	eng - English
Country	CH - Switzerland
Keywords	metal nanoparticles ; antimicrobial properties ; mesenchymal stem cells ; toxicity
OECD category	Public and environmental health
R&D Projects	GA21-17720S GA ČR - Czech Science Foundation (CSF)
	LM2018133 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	EF16_013/0001821 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LM2018124 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	UEM-P - RVO:68378041
UT WOS	000959873500001
EID SCOPUS	85151149941
DOI	10.3390/toxics11030253
Annotation	Some metal nanoparticles (NP) are characterized by antimicrobial properties with the potential to be used as alternative antibiotics. However, NP may negatively impact human organism, including mesenchymal stem cells (MSC), a cell population contributing to tissue growth and regeneration. To address these issues, we investigated the toxic effects of selected NP (Ag, ZnO, and CuO) in mouse MSC. MSC were treated with various doses of NP for 4 h, 24 h, and 48 h and multiple endpoints were analyzed. Reactive oxygen species were generated after 48 h CuO NP exposure. Lipid peroxidation was induced after 4 h and 24 h treatment, regardless of NP and/or tested dose. DNA fragmentation and oxidation induced by Ag NP showed dose responses for all the periods. For other NP, the effects were observed for shorter exposure times. The impact on the frequency of micronuclei was weak. All the tested NP increased the sensitivity of MSC to apoptosis. The cell cycle was most affected after 24 h, particularly for Ag NP treatment. In summary, the tested NP induced numerous adverse changes in MSC. These results should be taken into consideration when planning the use of NP in medical applications where MSC are involved.
Workplace	Institute of Experimental Medicine
Contact	Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218
Year of Publishing	2024
Electronic address	https://www.mdpi.com/2305-6304/11/3/253

Number of the records: 1