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Microfluidic platform for cell lysis: Towards single-molecule analysis in a free solution
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SYSNO ASEP 0576903 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Microfluidic platform for cell lysis: Towards single-molecule analysis in a free solution Author(s) Křivánková, Jana (UIACH-O) RID, ORCID
Hlaváček, Antonín (UIACH-O) ORCIDNumber of authors 2 Source Title 8th CASEAN Proceedings. - Vinh City : Publishing House for Science and Technology, 2023 - ISBN 978-604-357-225-4 Pages s. 34-40 Number of pages 7 s. Publication form Online - E Action The Academic Conference On Natural Science For Young Scientists, Master And PhD Students From Asean Countries /8./ Event date 27.08.2023 - 30.08.2023 VEvent location Vinh City Country VN - Viet Nam Event type WRD Language eng - English Country VN - Viet Nam Keywords droplet microfluidics ; polydimethylsiloxane chip ; cell lysis Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GA21-03156S GA ČR - Czech Science Foundation (CSF) Institutional support UIACH-O - RVO:68081715 Annotation We proposed a PDMS droplet-based microfluidic chip, with passive cell encapsulation, passive mixing, and incubation part. Discrete droplets with at least one cell per droplet in a regulated fashion of about 1Hz were formed and the cell-lysis concept using detergent Triton X-100 was optimized. The examined approach has essential benefits for cell manipulation, separation, and analysis of the droplet content. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2024 Electronic address https://hdl.handle.net/11104/0346292
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