Rutinosidase and other diglycosidases: Rising stars in biotechnology

0574998 - MBÚ 2024 RIV GB eng J - Journal Article
Křen, Vladimír - Bojarová, Pavla
Rutinosidase and other diglycosidases: Rising stars in biotechnology.
Biotechnology Advances. Roč. 68, Nov 2023 (2023), č. článku 108217. ISSN 0734-9750. E-ISSN 1873-1899
R&D Projects: GA ČR(CZ) GF22-00197K; GA MŠMT(CZ) LTC20069; EC(CZ) CA18132
Institutional support: RVO:61388971
Keywords : Diglycosidase * Glycobiology * Primeverosidase * Rutinosidase * Transglycosylation
OECD category: Biochemistry and molecular biology
Impact factor: 16, year: 2022
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0734975023001246?via%3Dihub

Diglycosidases are a special class of glycosidases (EC 3.2.1) that catalyze the separation of intact disaccharide moieties from the aglycone part. The main diglycosidase representatives comprise rutinosidases that cleave rutinose (& alpha,-L-Rha(1-6)-& beta,-D-Glc) from rutin or other rutinosides, and (iso)primeverosidases processing (iso)primeverosides (D-Xyl(1-6)-& beta,-D-Glc), but other activities are known. Notably, some diglycosidases may be ranked as monoglucosidases with enlarged substrate specificity. Diglycosidases are found in various microorganisms and plants. Diglycosidases are used in the food industry for aroma enhancement and flavor modification. Besides their hydrolytic activity, they also possess pronounced synthetic (transglycosylating) capabilities. Recently, they have been demonstrated to glycosylate various substrates in a high yield, including peculiar species like inorganic azide or carboxylic acids, which is a unique feature in biocatalysis. Rhamnose-containing compounds such as rutinose are currently receiving increased attention due to their proven activity in anti-cancer and dermatological experimental studies. This review demonstrates the vast and yet underrated biotechnological potential of diglycosidases from various sources (plant, microbial), and reveals perspectives on the use of these catalysts as well as of their products in biotechnology.
Permanent Link: https://hdl.handle.net/11104/0344874