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Isolation of Mycosporine-like Amino Acids from Red Macroalgae and a Marine Lichen by High-Performance Countercurrent Chromatography: A Strategy to Obtain Biological UV-Filters
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SYSNO ASEP 0573732 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Isolation of Mycosporine-like Amino Acids from Red Macroalgae and a Marine Lichen by High-Performance Countercurrent Chromatography: A Strategy to Obtain Biological UV-Filters Author(s) Vega, J. (CZ)
Bárcenas-Pérez, Daniela (MBU-M) ORCID, RID
Fuentes-Ríos, D. (ES)
López-Romero, J. M. (ES)
Hrouzek, Pavel (MBU-M) ORCID
Figueroa, F. L. (ES)
Cheel, José (MBU-M) RID, ORCIDArticle number 357 Source Title Marine Drugs. - : MDPI
Roč. 21, č. 6 (2023)Number of pages 17 s. Language eng - English Country CH - Switzerland Keywords countercurrent chromatography ; isolation ; marine lichen ; mycosporine-like amino acids ; photoprotection ; red macroalgae Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects TN01000048 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 001017315200001 EID SCOPUS 85163695862 DOI 10.3390/md21060357 Annotation Marine organisms have gained considerable biotechnological interest in recent years due to their wide variety of bioactive compounds with potential applications. Mycosporine-like amino acids (MAAs) are UV-absorbing secondary metabolites with antioxidant and photoprotective capacity, mainly found in organisms living under stress conditions (e.g., cyanobacteria, red algae, or lichens). In this work, five MAAs were isolated from two red macroalgae (Pyropia columbina and Gelidium corneum) and one marine lichen (Lichina pygmaea) by high-performance countercurrent chromatography (HPCCC). The selected biphasic solvent system consisted of ethanol, acetonitrile, saturated ammonium sulphate solution, and water (1:1:0.5:1, v:v:v:v). The HPCCC process for P. columbina and G. corneum consisted of eight separation cycles (1 g and 200 mg of extract per cycle, respectively), whereas three cycles were performed for of L. pygmaea (1.2 g extract per cycle). The separation process resulted in fractions enriched with palythine (2.3 mg), asterina-330 (3.3 mg), shinorine (14.8 mg), porphyra-334 (203.5 mg) and mycosporine-serinol (46.6 mg), which were subsequently desalted by using precipitation with methanol and permeation on a Sephadex G-10 column. Target molecules were identified by HPLC, MS, and NMR. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2024 Electronic address https://www.mdpi.com/1660-3397/21/6/357
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