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Mineralised polylactide and polycaprolactone soft foams with hierarchical micro-macro porous structure for tissue engineering
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SYSNO ASEP 0567467 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Mineralised polylactide and polycaprolactone soft foams with hierarchical micro-macro porous structure for tissue engineering Author(s) Beran, M. (CZ)
Berková, E. (CZ)
Musílková, Jana (FGU-C) RID, ORCID
Sedlář, Antonín (FGU-C) ORCID
Slepička, P. (CZ)
Fajstavr, D. (CZ)Source Title NANOCON 2021 - Conference proceedings. - Ostrava : Tanger Ltd., 2021 - ISSN 2694-930X - ISBN 978-80-88365-00-6 Pages s. 298-303 Number of pages 6 s. Publication form Online - E Action International Conference on Nanomaterials - Research & Application /13./ NANOCON Event date 20.10.2021 - 22.10.2021 VEvent location Brno Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords tissue engineering ; polylactide ; polycarolactone ; soft foams ; hierarchical structure OECD category Biomaterials (as related to medical implants, devices, sensors) R&D Projects NU20-08-00208 GA MZd - Ministry of Health (MZ) Institutional support FGU-C - RVO:67985823 EID SCOPUS 85127243695 DOI 10.37904/nanocon.2021.4346 Annotation The purpose of the study was development of soft foams from resorbable polymers with unique micro-macro porous interconnected hierarchical structure specially designed as scaffold for engineering of soft tissues. The foams have been prepared by freeze-drying of solutions of polylactide (PLA) and polycaprolactone (PCL) in 1,4-dioxane. The foams prepared by freeze-drying had interconnected porous aerogel characteristics. The hierarchical structure with bimodal micro-macro pore size distribution were obtained after addition of sucrose or NaCl porogens with defined crystal size distributions to the solutions before freeze-drying and leaching the porogen crystals from the freeze-dried foams with demineralised water. Polyethyleneimine was chemically conjugated to the alkali-treated foams followed by conjugation of citric acid using carbodiimide chemistry. Finally, they were mineralised by immersing and incubating in a simulated body fluid with ionic concentration similar to that of human blood plasma, to obtain tissue engineering scaffolds. To verify their biocompatibility, the scaffolds were seeded with adipose-derived stem cells (ASC) and sarcoma osteogenic-2 (SaOs-2) human osteoblast-like cells. Morphology of the cells attached to the scaffolds was evaluated and their viability was verified by a metabolic test. Biocompatibility and usability of the scaffolds was successfully verified by incubation with adipose-derived stem cells and SaOs-2 human osteosarcoma cell line. Mineralised scaffolds are more suitable growth supports for both the cell types than unmineralized collagen scaffolds. The scaffolds have been specially designed for engineering of soft tissues, but they can be used in other categories of tissue engineering, too. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2023 Electronic address https://www.confer.cz/nanocon/2021/read/4346-mineralised-polylactide-and-polycaprolactone-soft-foams-with-hierarchical-micro-macro-porous-structure-for-tissue-engineering.pdf
Number of the records: 1