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Detection of globular and fibrillar proteins by quartz crystal microbalance sensor coated with a functionalized diamond thin film
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SYSNO ASEP 0561903 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Detection of globular and fibrillar proteins by quartz crystal microbalance sensor coated with a functionalized diamond thin film Author(s) Procházka, Václav (FZU-D) ORCID
Kulha, P. (CZ)
Izsák, Tibor (FZU-D) ORCID
Ukraintsev, Egor (FZU-D) RID, ORCID
Varga, Marián (FZU-D) RID, ORCID
Jirásek, Vít (UFP-V)
Kromka, Alexander (FZU-D) RID, ORCID, SAINumber of authors 7 Article number 153017 Source Title Applied Surface Science. - : Elsevier - ISSN 0169-4332
Roč. 589, July (2022)Number of pages 8 s. Language eng - English Country NL - Netherlands Keywords NCD ; diamond ; QCM ; BSA ; FN ; biosensors ; functionalization Subject RIV BO - Biophysics OECD category Fluids and plasma physics (including surface physics) Subject RIV - cooperation Institute of Plasma Physics - Biotechnology ; Bionics R&D Projects EF16_019/0000760 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2018110 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support FZU-D - RVO:68378271 ; UFP-V - RVO:61389021 UT WOS 000780337200003 EID SCOPUS 85126027626 DOI 10.1016/j.apsusc.2022.153017 Annotation This study presents a sensor based on quartz crystal microbalance (QCM) coated with nanocrystalline diamond (NCD) thin film, functionalization method and novel application of such sensor. Diamond-coated QCMs (DQCMs) were superficially terminated by hydrogen and oxygen (H-NCD and O-NCD) to control its surface dipole/po- tential. Two protein solutions were tested: bovine serum albumin (BSA) and fibronectin (FN). We performed reference measurements of serial resonant frequency (SRF) of clean QCMs loaded with protein and compared them with SRF shifts DQCMs loaded with proteins. In order to investigate the influence of the deposited NCD thin film on QCM measuring capabilities, additional FEM analysis was performed. The simulation results showed that QCM sensors maintain the sensing capabilities with a rigid thin film of NCD on its surface. The shift of SRF was demonstrably caused by the weight of protein adhered to the diamond film’s surface. We compared masses estimated from the Sauerbrey equation to characterize the adhesive properties of the studied proteins. Comparing bare QCM and DQCM, we discovered diamonds enhance the sensing performance for proteins. At the same time, it saturates quickly with phosphate buffer saline used as a diluent solution for proteins. Results showed a significant increase in protein adhesion confirmed by the increase of the mass for both oxygen and hydrogen-terminated DQCMs. Moreover, a different time-dependent behaviour (i.e. different adsorption rate, degrees of physisorption and/or preference of the diamond surface functionalization) of the O-NCD and H-NCD QCMs was observed for BSA and FN proteins. In this meaning, we propose a schematic model which describes the detection principle of BSA and FN proteins on H- and O-terminated DQCM sensors. Finally, a simple proof of concept for using the functionalized diamond-coated sensors with current stimulation and EQCM (Electro- chemical Quartz Crystal Microbalance) is also proposed.
Workplace Institute of Physics Contact Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Year of Publishing 2023 Electronic address https://doi.org/10.1016/j.apsusc.2022.153017
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