Low-scale syringe-made synthesis of 15N-labeled oligonucleotides

0561240 - ÚOCHB 2023 RIV GB eng J - Journal Article
Brož, Břetislav - Tureček, F. - Marek, Aleš
Low-scale syringe-made synthesis of 15N-labeled oligonucleotides.
Journal of Labelled Compounds and Radiopharmaceuticals. Roč. 65, č. 12 (2022), s. 309-314. ISSN 0362-4803. E-ISSN 1099-1344
R&D Projects: GA MŠMT(CZ) LTAUSA19094
Institutional support: RVO:61388963
Keywords : 15N-labeled oligonucleotides * low-scale * phosphoramidite synthesis * syringe-made codon synthesis
OECD category: Analytical chemistry
Impact factor: 1.8, year: 2022
Method of publishing: Limited access
https://doi.org/10.1002/jlcr.4000

Fast and reasonable low-scale (200 nmol) syringe-made synthesis of N-15-labeled oligonucleotides representing DNA trinucleotide codons is communicated. All codons were prepared by solid-phase controlled pore glass synthesis column technique via the phosphoramidite method. Twenty-four labeled oligonucleotides covering the DNA genetic code alphabet were prepared using commercially available reagents and affordable equipment in a reasonably short period of time, with acceptable yields and purity for direct applications in mass spectrometry.
Permanent Link: https://hdl.handle.net/11104/0333924