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Structural Features and Immunomodulatory Effects of Water-Extractable Polysaccharides from Macrolepiota procera (Scop.) Singer
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SYSNO ASEP 0560738 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Structural Features and Immunomodulatory Effects of Water-Extractable Polysaccharides from Macrolepiota procera (Scop.) Singer Author(s) Georgiev, Y.N. (BG)
Vašíček, Ondřej (BFU-R) ORCID, RID
Dzhambazov, B.M. (BG)
Batsalova, T.G. (BG)
Denev, P. (BG)
Dobreva, L. I. (BG)
Danova, S. (BG)
Simova, S. (BG)
Wold, C. (BG)
Ognyanov, M.H. (BG)
Paulsen, B.S. (NO)
Krastanov, A. (BG)Number of authors 12 Article number 848 Source Title Journal of Fungi. - : MDPI
Roč. 8, č. 8 (2022)Number of pages 26 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords Macrolepiota procera ; polysaccharides ; nmr ; immunomodulatory activity ; inflammation ; prebiotic activity Subject RIV EE - Microbiology, Virology OECD category Microbiology Method of publishing Open access Institutional support BFU-R - RVO:68081707 UT WOS 000845616900001 EID SCOPUS 85137370670 DOI 10.3390/jof8080848 Annotation Macrolepiota procera (MP) is an edible mushroom used in the treatment of diabetes, hypertension and inflammation. However, the structure and biological effects of its polysaccharides (PSs) are unclear. This study investigates the structural features of a PS complex from MP (MP-PSC), its immunomodulatory activities and effects on probiotic and pathogenic bacteria. MP-PSC was obtained by boiling water, and PSs were characterized by 2D NMR spectroscopy. The immunomodulatory effects on blood and derived neutrophils, other leukocytes, and murine macrophages were studied by flow cytometry, chemiluminescence, spectrophotometry, and ELISA. The total carbohydrate content of MP-PSC was 74.2%, with glycogen occupying 36.7%, followed by beta-D-glucan, alpha-L-fuco-2-(1,6)-D-galactan, and beta-D-glucomannan. MP-PSC (200 mu g/mL) increased the number of CD14+ monocyte cells in the blood, after ex vivo incubation for 24 h. It dose-dependently (50-200 mu g/mL) activated the spontaneous oxidative burst of whole blood phagocytes, NO, and interleukin 6 productions in RAW264.7 cells. MP-PSC exhibited a low antioxidant activity and failed to suppress the oxidative burst and NO generation, induced by inflammatory agents. It (2.0%, w/v) stimulated probiotic co-cultures and hindered the growth and biofilm development of Escherichia coli, Streptococcus mutans and Salmonella enterica. MP PSs can be included in synbiotics to test their immunostimulating effects on compromised immune systems and gut health. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2023 Electronic address https://www.mdpi.com/2309-608X/8/8/848
Number of the records: 1