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Quantitative mapping of mercury and selenium in mushroom fruit bodies with laser ablation–inductively coupled plasma‑mass spectrometry
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SYSNO ASEP 0559944 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Quantitative mapping of mercury and selenium in mushroom fruit bodies with laser ablation–inductively coupled plasma‑mass spectrometry Author(s) Braeuer, S. (BE)
van Helden, T. (BE)
van Acker, T. (BE)
Leroux, O. (BE)
Van Der Straeten, D. (BE)
Verbeken, A. (BE)
Borovička, Jan (GLU-S) RID, SAI, ORCID
Vanhaecke, F. (BE)Source Title Analytical and Bioanalytical Chemistry. - : Springer - ISSN 1618-2642
Roč. 414, č. 25 (2022), s. 7517-7530Number of pages 14 s. Language eng - English Country DE - Germany Keywords Bioimaging ; Heavy metal ; Trace element ; Macrofungi ; LA-ICP-MS ; Quantification OECD category Analytical chemistry Method of publishing Open access Institutional support GLU-S - RVO:67985831 UT WOS 000836078900001 EID SCOPUS 85135590935 DOI 10.1007/s00216-022-04240-y Annotation This work describes the development of a novel method for quantitative mapping of Hg and Se in mushroom fruit body tissues with laser ablation coupled to inductively coupled plasma-mass spectrometry (LA-ICP-MS). Different parameters of the protocol for preparation of the standards used for quantification via external calibration were assessed, e.g., the dissolution temperature of gelatin standards and the addition of chitosan and ʟ-cysteine as additives to the gelatin-based calibration droplets to better match the sample matrix. While chitosan was not suited for this purpose, the presence of ʟ-cysteine considerably improved the figures of merit of the calibration, leading to limits of detection of 0.006 and 0.3 µg g−1 for Hg and Se, respectively, at a pixel size of 20 × 20 µm. Further, an in-house reference material, ideally suited for the validation of the method for application to mushroom samples, was successfully prepared from a paste of Boletus edulisi. The newly developed method was used to investigate the distribution of Hg and Se in tissue sections of five porcini mushroom individuals of three different species (Boletus edulis, Boletus aereus, and Boletus pinophilus) and one sample of a parasol mushroom (Macrolepiota procera). For one sample, additional areas were ablated at higher spatial resolution, with a laser spot size down to 5 µm, which allows a detailed investigation of the spatial distribution of Hg and Se in mushrooms. Workplace Institute of Geology Contact Jana Popelková, popelkova@gli.cas.cz, Sabina Janíčková, Tel.: 233 087 272 Year of Publishing 2023 Electronic address https://link.springer.com/article/10.1007/s00216-022-04240-y#citeas
Number of the records: 1