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Fatal affairs - conjugational transfer of a dinoflagellate- killing plasmid between marine Rhodobacterales
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SYSNO ASEP 0557609 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Fatal affairs - conjugational transfer of a dinoflagellate- killing plasmid between marine Rhodobacterales Author(s) Tomasch, Jurgen (MBU-M) ORCID, RID
Ringel, V. (DE)
Wang, H. (DE)
Freese, H. (DE)
Bartling, P. (DE)
Brinkmann, H. (DE)
Vollmers, J. (DE)
Jarek, M. (DE)
Wagner-Doebler, I. (DE)
Petersen, J. (DE)Article number 000787 Source Title Microbial Genomics. - : Microbiology Society - ISSN 2057-5858
Roč. 8, č. 3 (2022)Number of pages 12 s. Language eng - English Country GB - United Kingdom Keywords phaeobacter-gallaeciensis ; dinoroseobacter-shibae ; tropodithietic acid ; gen. nov. ; roseobacter ; genome ; phytoplankton ; adaptation ; bacteria ; agent ; bacteria-algae interaction ; conjugation ; horizontal gene transfer ; Roseobacter Subject RIV EE - Microbiology, Virology OECD category Microbiology Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000788985200003 EID SCOPUS 85125871952 DOI 10.1099/mgen.0.000787 Annotation The roseobacter group of marine bacteria is characterized by a mosaic distribution of ecologically important phenotypes. These are often encoded on mobile extrachromosomal replicons. So far, conjugation had only been experimentally proven between the two model organisms Phaeobacter inhibens and Dinoroseobacter shibae. Here, we show that two large natural RepABCtype plasmids from D. shibae can be transferred into representatives of all known major Rhodobacterales lineages. Complete genome sequencing of the newly established Phaeobacter inhibens transconjugants confirmed their genomic integrity. The conjugated plasmids were stably maintained as single copy number replicons in the genuine as well as the new host. Co- cultivation of Phaeobacter inhibens and the transconjugants with the dinoflagellate Prorocentrum minimum demonstrated that Phaeobacter inhibens is a probiotic strain that improves the yield and stability of the dinoflagellate culture. The transconjugant carrying the 191 kb plasmid, but not the 126 kb sister plasmid, killed the dinoflagellate in co- culture. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2023 Electronic address https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000787
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