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Application Potential of Cyanide Hydratase from Exidia glandulosa: Free Cyanide Removal from Simulated Industrial Effluents
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SYSNO ASEP 0549581 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Application Potential of Cyanide Hydratase from Exidia glandulosa: Free Cyanide Removal from Simulated Industrial Effluents Author(s) Sedova, Anastasia (MBU-M) ORCID
Rucká, Lenka (MBU-M)
Bojarová, Pavla (MBU-M) ORCID
Glozlová, Michaela (MBU-M)
Novotný, Petr (MBU-M) ORCID
Křístková, Barbora (MBU-M)
Pátek, Miroslav (MBU-M) RID, ORCID
Martínková, Ludmila (MBU-M) RID, ORCIDArticle number 1410 Source Title Catalysts. - : MDPI
Roč. 11, č. 11 (2021)Number of pages 16 s. Language eng - English Country CH - Switzerland Keywords biocatalyst ; cyanide hydratase ; nitrilase ; Exidia glandulosa ; industrial effluent ; cokemaking ; electroplating ; wastewater treatment ; free cyanide ; formamide Subject RIV EA - Cell Biology OECD category Microbiology R&D Projects GA18-00184S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000723298100001 EID SCOPUS 85119371203 DOI 10.3390/catal11111410 Annotation Industries such as mining, cokemaking, (petro)chemical and electroplating produce effluents that contain free cyanide (fCN = HCN + CN-). Currently, fCN is mainly removed by (physico)chemical methods or by biotreatment with activated sludge. Cyanide hydratases (CynHs) (EC 4.2.1.66), which convert fCN to the much less toxic formamide, have been considered for a mild approach to wastewater decyanation. However, few data are available to evaluate the application potential of CynHs. In this study, we used a new CynH from Exidia glandulosa (protein KZV92691.1 designated NitEg by us), which was overproduced in Escherichia coli. The purified NitEg was highly active for fCN with 784 U/mg protein, k(cat) 927/s and k(cat)/K-M 42/s/mM. It exhibited optimal activities at pH approximately 6-9 and 40-45 & DEG,C. It was quite stable in this pH range, and retained approximately 40% activity at 37 & DEG,C after 1 day. Silver and copper ions (1 mM) decreased its activity by 30-40%. The removal of 98-100% fCN was achieved for 0.6-100 mM fCN. Moreover, thiocyanate, sulfide, ammonia or phenol added in amounts typical of industrial effluents did not significantly reduce the fCN conversion, while electroplating effluents may need to be diluted due to high fCN and metal content. The ease of preparation of NitEg, its high specific activity, robustness and long shelf life make it a promising biocatalyst for the detoxification of fCN. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://www.mdpi.com/2073-4344/11/11/1410
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