Number of the records: 1
p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice
- 1.
SYSNO ASEP 0543951 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice Author(s) Bora, P. (CZ)
Gahurová, Lenka (UZFG-Y) ORCID
Mašek, T. (CZ)
Hauserová, A. (CZ)
Potěšil, D. (CZ)
Jansová, Denisa (UZFG-Y) ORCID
Šušor, Andrej (UZFG-Y) RID, ORCID
Zdráhal, Z. (CZ)
Ajduk, A. (CZ)
Pospíšek, M. (CZ)
Bruce, A. W. (CZ)Article number 788 Source Title Communications Biology. - : Nature Publishing Group
Roč. 4, č. 1 (2021)Number of pages 19 s. Publication form Online - E Language eng - English Country GB - United Kingdom Keywords early blastocyst development ; mouse Subject RIV EB - Genetics ; Molecular Biology OECD category Developmental biology Method of publishing Open access Institutional support UZFG-Y - RVO:67985904 UT WOS 000668739100001 EID SCOPUS 85111784798 DOI 10.1038/s42003-021-02290-z Annotation Successful specification of the two mouse blastocyst inner cell mass (ICM) lineages (the primitive endoderm (PrE) and epiblast) is a prerequisite for continued development and requires active fibroblast growth factor 4 (FGF4) signaling. Previously, we identified a role for p38 mitogen-activated protein kinases (p38-MAPKs) during PrE differentiation, but the underlying mechanisms have remained unresolved. Here, we report an early blastocyst window of p38-MAPK activity that is required to regulate ribosome-related gene expression, rRNA precursor processing, polysome formation and protein translation. We show that p38-MAPK inhibition-induced PrE phenotypes can be partially rescued by activating the translational regulator mTOR. However, similar PrE phenotypes associated with extracellular signal-regulated kinase (ERK) pathway inhibition targeting active FGF4 signaling are not affected by mTOR activation. These data indicate a specific role for p38-MAPKs in providing a permissive translational environment during mouse blastocyst PrE differentiation that is distinct from classically reported FGF4-based mechanisms. Bora et al. show that an early blastocyst window of p38-MAPK activity regulates ribosome-related gene expression, rRNA precursor processing, polysome formation, and protein translation. This study suggests a distinct role of p38-MAPKs for providing a permissive translational environment during mouse blastocyst primitive endoderm differentiation. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2022 Electronic address https://www.nature.com/articles/s42003-021-02290-z
Number of the records: 1