Number of the records: 1
Micellar electrokinetic chromatography as a powerful analytical tool for research on prebiotic chemistry
- 1.
SYSNO ASEP 0543635 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Micellar electrokinetic chromatography as a powerful analytical tool for research on prebiotic chemistry Author(s) Zemánková, K. (CZ)
Nejdl, L. (CZ)
Bezděková, J. (CZ)
Vodová, M. (CZ)
Petera, Lukáš (UFCH-W) ORCID
Pastorek, Adam (UFCH-W) ORCID
Civiš, Svatopluk (UFCH-W) RID, ORCID, SAI
Kubelík, Petr (UFCH-W) RID, ORCID
Ferus, Martin (UFCH-W) ORCID, RID
Adam, V. (CZ)
Vaculovičová, M. (CZ)Article number 106022 Source Title Microchemical Journal. - : Elsevier - ISSN 0026-265X
Roč. 167, AUG 2021 (2021)Number of pages 7 s. Language eng - English Country US - United States Keywords high-energy chemistry ; capillary-electrophoresis ; rich atmospheres ; hydrogen-cyanide ; formamide ; origin ; purine ; bases ; nucleobases ; adenine ; Capillary electrophoresis ; Purine ; Pyrimidine ; Nucleobases ; Origin of life ; Molecularly imprinted polymers Subject RIV CF - Physical ; Theoretical Chemistry OECD category Physical chemistry R&D Projects GA19-03314S GA ČR - Czech Science Foundation (CSF) EF16_019/0000778 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support UFCH-W - RVO:61388955 UT WOS 000662640500005 DOI 10.1016/j.microc.2021.106022 Annotation Capillary electromigration techniques have proven their capabilities in detection of variety of analytes from inorganic ions and small organic molecules through bio(macro)molecules to large analytes such as cells or nano/ micro particles. Also broad range of potential applications includes food and environmental analysis, biomedical and pharmaceutical investigations or even diagnostics.
In this work, it was demonstrated that capillary micellar electrokinetic chromatography is an excellent tool
extremely helpful in investigations focused on prebiotic synthesis of molecules essential for formation of life on
early Earth – nucleobases. In particular, rapid separation of nucleobases (< 2 min) was achieved in 40 mM borate
buffer separation electrolyte containing 60 mM sodium dodecyl sulfate as an additive. This approach enabled
detection of nucleobases formed in thermolysed formamide under conditions simulating the environment
occurring on early Earth. Moreover, polydopamine-based molecularly imprinted polymers specific to thymine
and uracil improved detection of these low abundant products.Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2022 Electronic address http://hdl.handle.net/11104/0320824
Number of the records: 1