Enzymatically cross-linked hydrogels based on synthetic poly(.alpha.-amino acid)s functionalized with RGD peptide for 3D mesenchymal stem cell culture

0541734 - ÚMCH 2022 RIV US eng J - Journal Article
Dvořáková, Jana - Trousil, Jiří - Podhorská, Bohumila - Mikšovská, Zuzana - Janoušková, Olga - Proks, Vladimír
Enzymatically cross-linked hydrogels based on synthetic poly(.alpha.-amino acid)s functionalized with RGD peptide for 3D mesenchymal stem cell culture.
Biomacromolecules. Roč. 22, č. 4 (2021), s. 1417-1431. ISSN 1525-7797. E-ISSN 1526-4602
R&D Projects: GA ČR(CZ) GA18-03224S
Institutional support: RVO:61389013
Keywords : poly(α-amino acid) * enzymatic cross-linking * enzymatic degradation
OECD category: Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
Impact factor: 6.979, year: 2021
Method of publishing: Limited access
https://pubs.acs.org/doi/10.1021/acs.biomac.0c01641

Injectable hydrogel scaffolds combined with stem cell therapy represent a promising approach for minimally invasive surgical tissue repair. In this study, we developed and characterized a fully synthetic, biodegradable poly(N5-(2-hydroxyethyl)-l-glutamine)-based injectable hydrogel modified with integrin-binding arginine–glycine–aspartic acid (RGD) peptide (PHEG-Tyr-RGD). The biodegradable hydroxyphenyl polymer precursor derivative of PHEG-Tyr was enzymatically cross-linked to obtain injectable hydrogels with different physicochemical properties. The gelation time, gel yield, swelling behavior, and storage modulus of the PHEG-Tyr hydrogels were tuned by varying the concentrations of the PHEG-Tyr precursors and horseradish peroxidase as well as the nH2O2/nTyr ratio. The mechanical properties and gelation time of the PHEG-Tyr hydrogel were optimized for the encapsulation of rat mesenchymal stem cells (rMSCs). We focused on the 2D and 3D spreading and viability of rMSCs within the PHEG-Tyr-RGD hydrogels with different physicochemical microenvironments in vitro. Encapsulation of rMSCs shows long-term survival and exhibits cell–matrix and cell–cell interactions reflective of both the RGD concentration and hydrogel stiffness. The presented biomaterial represents a suitable biological microenvironment to guide 3D spreading and may act as a promising 3D artificial extracellular matrix for stem cell therapy.
Permanent Link: http://hdl.handle.net/11104/0320176