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Communicating macropores in PHEMA-based hydrogels for cell seeding: probabilistic open pore simulation and direct micro-CT proof
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SYSNO ASEP 0535213 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Communicating macropores in PHEMA-based hydrogels for cell seeding: probabilistic open pore simulation and direct micro-CT proof Author(s) Dušková-Smrčková, Miroslava (UMCH-V) RID
Zavřel, J. (CZ)
Bartoš, M. (CZ)
Kaberova, Zhansaya (UMCH-V) RID
Filová, Elena (FGU-C) RID, ORCID
Zárubová, Jana (FGU-C) RID, ORCID
Šlouf, Miroslav (UMCH-V) RID, ORCID
Michálek, Jiří (UMCH-V) RID, ORCID
Vampola, T. (CZ)
Kubies, Dana (UMCH-V) RID, ORCIDArticle number 109312 Source Title Materials and Design. - : Elsevier - ISSN 0264-1275
Roč. 198, 15 January (2021)Number of pages 17 s. Language eng - English Country GB - United Kingdom Keywords hydrogel ; simulation ; open macroporosity Subject RIV CD - Macromolecular Chemistry OECD category Polymer science Subject RIV - cooperation Institute of Physiology - Biotechnology ; Bionics R&D Projects GA17-08531S GA ČR - Czech Science Foundation (CSF) GA20-01570S GA ČR - Czech Science Foundation (CSF) LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UMCH-V - RVO:61389013 ; FGU-C - RVO:67985823 UT WOS 000607549100007 EID SCOPUS 85097233064 DOI 10.1016/j.matdes.2020.109312 Annotation Open macroporosity is crucial for scaffolds in tissue engineering. Porogen-templating method is an attractive approach for fabrication of macroporous hydrogels, however, the effect of shape and amount of template particles on imprinted structure has not yet been quantitatively established. We present a mathematical model for simulating the formation of paths percolating through distributed cubical particles as a function of the filling volume. The model was used to select the fraction of NaCl particles as templates for preparation of hydrogels with communicating pores. Hydrogels were prepared from 2-hydroxyethyl methacrylate (HEMA) copolymerized with 2-ethoxyethyl methacrylate (EOEMA), [2-methacryloyloxy)ethyl]trimethylammonium chloride (MOETACl) or ionizable methacrylic acid (MANa) to modulate swelling, surface and mechanical properties of gels. Micro-CT analysis of swollen samples proved a highly-interconnected pore structure. Charged hydrogels swelled more and their apparent elastic modulus G′ was below 1 kPa. For PHEMA and P(HEMA/EOEMA) hydrogels, G′ was 5 and 80 kPa, respectively. Within two-week in vitro studies, MG63 osteoblasts proliferated fastest on P(HEMA/EOEMA) showing the lowest swelling and the highest elastic modulus, whereas cell growth was impaired on positively charged P(HEMA/MOETACl). The mathematical simulation of cubical particle packing in hydrogels and micro-CT data in swollen state provided evidence of an extensive void communication in 3D. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2022 Electronic address https://www.sciencedirect.com/science/article/pii/S0264127520308480?via%3Dihub
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