DNA damage triggers reprogramming of differentiated cells into stem cells in Physcomitrella

0532864 - ÚEB 2021 RIV GB eng J - Journal Article
Gu, N. - Tamada, Y. - Imai, A. - Palfalvi, G. - Kabeya, Y. - Shigenobu, S. - Ishikawa, M. - Angelis, Karel - Chen, C. - Hasebe, M.
DNA damage triggers reprogramming of differentiated cells into stem cells in Physcomitrella.
Nature Plants. Roč. 6, č. 9 (2020), s. 1098-1105. ISSN 2055-026X. E-ISSN 2055-0278
R&D Projects: GA MŠMT LTC17047
Institutional support: RVO:61389030
Keywords : 2-TAILED COMET ASSAY * MOSS * REGENERATION
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 15.793, year: 2020
Method of publishing: Open access
http://doi.org/10.1038/s41477-020-0745-9

DNA damage can result from intrinsic cellular processes and from exposure to stressful environments. Such DNA damage generally threatens genome integrity and cell viability1. However, here we report that the transient induction of DNA strand breaks (single-strand breaks, double-strand breaks or both) in the moss Physcomitrella patens can trigger the reprogramming of differentiated leaf cells into stem cells without cell death. After intact leafy shoots (gametophores) were exposed to zeocin, an inducer of DNA strand breaks, the STEMCELL-INDUCING FACTOR 1 (STEMIN1)2 promoter was activated in some leaf cells. These cells subsequently initiated tip growth and underwent asymmetric cell divisions to form chloronema apical stem cells, which are in an earlier phase of the life cycle than leaf cells and have the ability to form new gametophores. This DNA-strand-break-induced reprogramming required the DNA damage sensor ATR kinase, but not ATM kinase, together with STEMIN1 and closely related proteins. ATR was also indispensable for the induction of STEMIN1 by DNA strand breaks. Our findings indicate that DNA strand breaks, which are usually considered to pose a severe threat to cells, trigger cellular reprogramming towards stem cells via the activity of ATR and STEMINs.
Permanent Link: http://hdl.handle.net/11104/0311245