Competitive upconversion-linked immunoassay using peptide mimetics for the detection of the mycotoxin zearalenone

0532838 - ÚIACH 2021 RIV NL eng J - Journal Article
Peltomaa, R. - Farka, Z. - Mickert, M. J. - Brandmeier, J. C. - Pastucha, M. - Hlaváček, Antonín - Martínez-Orts, M. - Canales, Á. - Skládal, P. - Benito-Peña, E. - Moreno-Bondi, M. C. - Gorris, H. H.
Competitive upconversion-linked immunoassay using peptide mimetics for the detection of the mycotoxin zearalenone.
Biosensors and Bioelectronics. Roč. 170, DEC (2020), s. 1-9, č. článku 112683. ISSN 0956-5663. E-ISSN 1873-4235
R&D Projects: GA ČR(CZ) GJ18-03367Y
Institutional support: RVO:68081715
Keywords : zearalenone * upconversion nanoparticle * immunosensing * surface plasmon resonance
OECD category: Analytical chemistry
Impact factor: 10.618, year: 2020
Method of publishing: Limited access

Due to increasing food safety standards, the analysis of mycotoxins has become essential in the food industry. In this work, we have developed a competitive upconversion-linked immunosorbent assay (ULISA) for the analysis of zearalenone (ZEA), one of the most frequently encountered mycotoxins in food worldwide. Instead of a toxin-conjugate conventionally used in competitive immunoassays, we designed a ZEA mimicking peptide extended by a biotin-linker and confirmed its excellent suitability to
mimic ZEA by nuclear magnetic resonance (NMR) and surface plasmon resonance (SPR) analysis. Upconversion nanoparticles (UCNP, type NaYF4:Yb,Tm) served as background-free optical label for the detection of the peptide mimetic in the competitive ULISA. Streptavidin-conjugated UCNPs were prepared by click reaction using an alkyne-PEG-neridronate linker. The UCNP conjugate clearly outperformed conventional labels such as enzymes or fluorescent dyes. With a limit of detection of 20 pg mL−1 (63 pM), the competitive ULISA is well applicable to the detection of ZEA at the levels set by the European legislation. Moreover, the ULISA is specific for ZEA and its metabolites (α- and β- zearalenol) without significant cross-reactivity with other related mycotoxins. We detected ZEA in spiked and naturally contaminated maize samples using liquid chromatography–tandem mass spektrometry (UPLC-MS/MS) as a reference method to demonstrate food analysis in real samples.
Permanent Link: http://hdl.handle.net/11104/0311222