Number of the records: 1
Simultaneous label-free live imaging of cell nucleus and luminescent nanodiamonds
- 1.
SYSNO ASEP 0531479 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Simultaneous label-free live imaging of cell nucleus and luminescent nanodiamonds Author(s) Gulka, M. (BE)
Salehi, H. (FR)
Varga, B. (FR)
Middendorp, E. (FR)
Pall, O. (FR)
Raabová, Helena (UOCHB-X)
Cloitre, T. (FR)
Cuisinier, F. J. G. (FR)
Cígler, Petr (UOCHB-X) RID, ORCID
Nesládek, M. (BE)
Gergely, C. (FR)Article number 9791 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 10, Jun 17 (2020)Number of pages 9 s. Language eng - English Country GB - United Kingdom Keywords fluorescent nanodiamonds ; Raman spectroscopy ; mass production ; DNA Subject RIV CD - Macromolecular Chemistry OECD category Nano-materials (production and properties) R&D Projects GA16-16336S GA ČR - Czech Science Foundation (CSF) EF16_019/0000729 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_026/0008382 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UOCHB-X - RVO:61388963 UT WOS 000543956500031 EID SCOPUS 85086739076 DOI 10.1038/s41598-020-66593-7 Annotation In recent years, fluorescent nanodiamond (fND) particles containing nitrogen-vacancy (NV) centers gained recognition as an attractive probe for nanoscale cellular imaging and quantum sensing. For these applications, precise localization of fNDs inside of a living cell is essential. Here we propose such a method by simultaneous detection of the signal from the NV centers and the spectroscopic Raman signal from the cells to visualize the nucleus of living cells. However, we show that the commonly used Raman cell signal from the fingerprint region is not suitable for organelle imaging in this case. Therefore, we develop a method for nucleus visualization exploiting the region-specific shape of C-H stretching mode and further use k-means cluster analysis to chemically distinguish the vicinity of fNDs. Our technique enables, within a single scan, to detect fNDs, distinguish by chemical localization whether they have been internalized into cell and simultaneously visualize cell nucleus without any labeling or cell-fixation. We show for the first time spectral colocalization of unmodified high-pressure high-temperature fND probes with the cell nucleus. Our methodology can be, in principle, extended to any red- and near-infrared-luminescent cell-probes and is fully compatible with quantum sensing measurements in living cells. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2021 Electronic address https://doi.org/10.1038/s41598-020-66593-7
Number of the records: 1