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The high-resolution proteomic analysis of protein composition of rat spleen lymphocytes stimulated by Concanavalin A, a comparison with morphine-treated cells
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SYSNO ASEP 0523837 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The high-resolution proteomic analysis of protein composition of rat spleen lymphocytes stimulated by Concanavalin A, a comparison with morphine-treated cells Author(s) Ujčíková, Hana (FGU-C) RID, ORCID
Cechová, Kristína (FGU-C) RID, ORCID
Roubalová, Lenka (FGU-C) RID, ORCID, SAI
Brejchová, Jana (FGU-C) RID, ORCID
Kaufman, Jonáš (FGU-C)
Holáň, Vladimír (UEM-P) RID
Svoboda, Petr (FGU-C) RID, ORCIDArticle number 577191 Source Title Journal of Neuroimmunology. - : Elsevier - ISSN 0165-5728
Roč. 341, Apr 15 (2020)Number of pages 14 s. Language eng - English Country NL - Netherlands Keywords morphine ; concanavalin A ; rat spleen lymphocytes ; proteomic analysis ; up-regulation of brain proteins Subject RIV ED - Physiology OECD category Physiology (including cytology) Subject RIV - cooperation Institute of Experimental Medicine - Genetics ; Molecular Biology R&D Projects GA19-03295S GA ČR - Czech Science Foundation (CSF) LTAUSA18110 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support FGU-C - RVO:67985823 ; UEM-P - RVO:68378041 UT WOS 000521114100009 EID SCOPUS 85079849005 DOI 10.1016/j.jneuroim.2020.577191 Annotation Morphine- and Concanavalin A-induced changes of protein composition of rat spleen lymphocytes were determined by high-resolution proteomic analysis, gel-free, label-free quantification, MaxLFQ. Stimulation by Con A resulted in a major reorganization of spleen cell protein composition evidenced by increased expression level of 94 proteins, 101 proteins were down-regulated (> 2-fold). Interestingly, among proteins that were up-regulated to the largest extent were the prototypical brain proteins as a neuron specific enolase, synapsin-1, brain acid-soluble protein-1 and myelin basic protein. Morphine-induced change was limited to no more than 5 up-regulated and 18 down-regulated proteins (> 2-fold). Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2021 Electronic address https://doi.org/10.1016/j.jneuroim.2020.577191
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