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Flow cytometric characterisation of the complex polyploid genome of Saccharum officinarum and modern sugarcane cultivars
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SYSNO ASEP 0522098 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Flow cytometric characterisation of the complex polyploid genome of Saccharum officinarum and modern sugarcane cultivars Author(s) Metcalfe, C. J. (AU)
Li, J. (CN)
Giorgi, D. (IT)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Piperidis, N. (AU)
Aitken, K. S. (AU)Number of authors 6 Article number 19362 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 9, DEC 18 (2019)Number of pages 12 s. Language eng - English Country GB - United Kingdom Keywords MAP ; IDENTIFICATION ; CHROMOSOMES Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000507594500002 EID SCOPUS 85076886234 DOI 10.1038/s41598-019-55652-3 Annotation Sugarcane (Saccharum spp.) is a globally important crop for sugar and bioenergy production. Its highly polyploid, complex genome has hindered progress in understanding its molecular structure. Flow cytometric sorting and analysis has been used in other important crops with large genomes to dissect the genome into component chromosomes. Here we present for the first time a method to prepare suspensions of intact sugarcane chromosomes for flow cytometric analysis and sorting. Flow karyotypes were generated for two S. officinarum and three hybrid cultivars. Five main peaks were identified and each genotype had a distinct flow karyotype profile. The flow karyotypes of S. officinarum were sharper and with more discrete peaks than the hybrids, this difference is probably due to the double genome structure of the hybrids. Simple Sequence Repeat (SSR) markers were used to determine that at least one allelic copy of each of the 10 basic chromosomes could be found in each peak for every genotype, except R570, suggesting that the peaks may represent ancestral Saccharum sub genomes. The ability to flow sort Saccharum chromosomes will allow us to isolate and analyse chromosomes of interest and further examine the structure and evolution of the sugarcane genome. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2020 Electronic address https://www.nature.com/articles/s41598-019-55652-3
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