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Tuftelin and HIFs expression in osteogenesis
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SYSNO ASEP 0517325 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Tuftelin and HIFs expression in osteogenesis Author(s) Bobek, Jan (UZFG-Y) ORCID
Oralová, Veronika (UZFG-Y) RID, ORCID
Kratochvílová, Adéla (UZFG-Y) ORCID
Žváčková, Ivana (UZFG-Y)
Lesot, Hervé (UZFG-Y)
Matalová, Eva (UZFG-Y) RIDSource Title Histochemistry and Cell Biology. - : Springer - ISSN 0948-6143
Roč. 152, č. 5 (2019), s. 355-363Number of pages 9 s. Publication form Print - P Language eng - English Country DE - Germany Keywords intramembranous ; ossification ; bone Subject RIV EA - Cell Biology OECD category Developmental biology R&D Projects GA17-14886S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support UZFG-Y - RVO:67985904 UT WOS 000495293400005 EID SCOPUS 85073792103 DOI 10.1007/s00418-019-01813-4 Annotation Tuftelin was originally discovered and mostly studied in the tooth, but later found also in other organs. Despite its wide distribution among tissues, tuftelin's function has so far been specified only in the formation of enamel crystals. Nevertheless, in many cases, tuftelin was suggested to be associated with cellular adaptation to hypoxia and recently even with cell differentiation. Therefore, we aimed to investigate tuftelin expression along with hypoxia-inducible factors (HIFs) during the early development of the mandibular/alveolar (m/a) bone, when osteoblasts started to differentiate in vivo and to compare their expression levels in undifferentiated versus differentiated osteoblastic cells in vitro. Immunohistochemistry demonstrated the presence of tuftelin already in osteoblastic precursors which were also HIF1-positive, but HIF2-negative. Nevertheless, HIF2 protein appeared when osteoblasts differentiated, one day later. This is in agreement with observations made with MC3T3-E1 cells, where there was no significant difference in tuftelin and Hif1 expression in undifferentiated vs. differentiated cells, although Hif2 increased upon differentiation induction. In differentiated osteoblasts of the m/a bone, all three proteins accumulated, first, prenatally, in the cytoplasm and later, particularly at postnatal stages, they displayed also peri/nuclear localization. Such a dynamic time-space pattern of tuftelin expression has recently been reported in neurons, which, as the m/a bone, differentiate under less hypoxic conditions as indicated also by a prevalent cytoplasmic expression of HIF1 in osteoblasts. However, unlike what was shown in cultured neurons, tuftelin does not seem to participate in final osteoblastic differentiation and its functions, thus, appears to be tissue specific. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2020 Electronic address https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=78905713317
Number of the records: 1