Differentiation Induction as a Response to Irradiation in Neural Stem Cells In Vitro

0511740 - ÚMG 2020 RIV CH eng J - Journal Article
Konířová, Jana - Cupal, I. - Jarošová, Šárka - Michaelidesova, A. - Vachlova, J. - Davídková, M. - Bartůněk, Petr - Zíková, Martina
Differentiation Induction as a Response to Irradiation in Neural Stem Cells In Vitro.
Cancers (Basel). Roč. 11, č. 7 (2019), č. článku 913. E-ISSN 2072-6694
R&D Projects: GA MŠMT LO1419
Institutional support: RVO:68378050
Keywords : neural stem cells * ionizing radiation * proliferation * proliferative arrest * differentiation * apoptosis
OECD category: Cell biology
Impact factor: 6.126, year: 2019
Method of publishing: Open access
https://www.mdpi.com/2072-6694/11/7/913

Radiotherapy plays a significant role in brain cancer treatment, however, the use of this therapy is often accompanied by neurocognitive decline that is, at least partially, a consequence of radiation-induced damage to neural stem cell populations. Our findings describe features that define the response of neural stem cells (NSCs) to ionizing radiation. We investigated the effects of irradiation on neural stem cells isolated from the ventricular-subventricular zone of mouse brain and cultivated in vitro. Our findings describe the increased transcriptional activity of p53 targets and proliferative arrest after irradiation. Moreover, we show that most cells do not undergo apoptosis after irradiation but rather cease proliferation and start a differentiation program. Induction of differentiation and the demonstrated potential of irradiated cells to differentiate into neurons may represent a mechanism whereby damaged NSCs eliminate potentially hazardous cells and circumvent the debilitating consequences of cumulative DNA damage.
Permanent Link: http://hdl.handle.net/11104/0302107