Mechanisms of sublethal copper toxicity damage to the photosynthetic apparatus of Rhodospirillum rubrum

Jaime-Perez, Noelia; Kaftan, David; Bína, David; Bokhari, Syed Nadeem Hussain; Shreedhar, Sowmya; Küpper, Hendrik

doi:https://dx.doi.org/10.1016/j.bbabio.2019.06.004

Number of the records: 1

Mechanisms of sublethal copper toxicity damage to the photosynthetic apparatus of Rhodospirillum rubrum

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SYSNO ASEP	0511086
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Mechanisms of sublethal copper toxicity damage to the photosynthetic apparatus of Rhodospirillum rubrum
Author(s)	Jaime-Perez, Noelia (BC-A) Kaftan, David (MBU-M)_ORCID Bína, David (BC-A)_{RID, ORCID} Bokhari, Syed Nadeem Hussain (BC-A)_RID Shreedhar, Sowmya (BC-A) Küpper, Hendrik (BC-A)_{RID, ORCID}
Number of authors	6
Source Title	Biochimica Et Biophysica Acta-Bioenergetics. - : Elsevier - ISSN 0005-2728 Roč. 1860, č. 8 (2019), s. 640-650
Number of pages	11 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Bacteriochlorophyll ; Purple bacteria ; Copper toxicity ; Light harvesting complex ; Photosynthesis
Subject RIV	CE - Biochemistry
OECD category	Biochemistry and molecular biology
Subject RIV - cooperation	Institute of Microbiology - Biochemistry
R&D Projects	EF15_003/0000336 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA17-14024S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Open access
Institutional support	BC-A - RVO:60077344 ; MBU-M - RVO:61388971
UT WOS	000480668900005
EID SCOPUS	85068623556
DOI	10.1016/j.bbabio.2019.06.004
Annotation	Magnesium (Mg2+) is the ubiquitous metal ion present in chlorophyll and bacteriochlorophyll (BChl), involved in photosystems in photosynthetic organisms. In the present study we investigated targets of toxic copper binding to the photosynthetic apparatus of the anoxygenic purple bacterium Rhodospirillum rubrum. This was done by a combination of in vivo measurements of flash photolysis and fast fluorescence kinetics combined with the analysis of metal binding to pigments and pigment-protein complexes isolated from Cu-stressed cells by HPLC-ICPMS (ICP-sfMS). This work concludes that R. rubrum is highly sensitive to Cu2+, with a strong inhibition of the photosynthetic reaction centres (RCs) already at 2 mu M Cu2+. The inhibition of growth and of RC activity was related to the formation of Cu-containing BChl degradation products that occurred much more in the RC than in LH1. These results suggest that the shift of metal centres in BChl from Mg2+ to Cu2+ can occur in vivo in the RCs of R. rubrum under environmentally realistic Cu2+ concentrations, leading to a strong inhibition of the function of these RCs.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2020
Electronic address	https://www.sciencedirect.com/science/article/pii/S0005272819300568?via%3Dihub

Number of the records: 1