Number of the records: 1
Application of Advanced Microscopic Methods to Study the Interaction of Carboxylated Fluorescent Nanodiamonds with Membrane Structures in THP-1 Cells: Activation of Inflammasome NLRP3 as the Result of Lysosome Destabilization
- 1.
SYSNO ASEP 0509040 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Application of Advanced Microscopic Methods to Study the Interaction of Carboxylated Fluorescent Nanodiamonds with Membrane Structures in THP-1 Cells: Activation of Inflammasome NLRP3 as the Result of Lysosome Destabilization Author(s) Knötigová, P.T. (CZ)
Mašek, J. (CZ)
Hubatka, F. (CZ)
Kotouček, J. (CZ)
Kulich, P. (CZ)
Šimečková, P. (CZ)
Bartheldyová, E. (CZ)
Machala, M. (CZ)
Švadláková, T. (CZ)
Krejsek, J. (CZ)
Vaškovicová, Naděžda (UPT-D) RID, ORCID, SAI
Skoupý, Radim (UPT-D) RID, ORCID, SAI
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
Macaulay, V. (GB)
Katzuba, M. (GB)
Fekete, Ladislav (FZU-D) RID, ORCID
Ashcheulov, Petr (FZU-D) ORCID, RID
Raška, M. (CZ)
Kratochvílová, Irena (FZU-D) RID, ORCID, SAI
Turánek, J. (CZ)Number of authors 20 Source Title Molecular Pharmaceutics. - : American Chemical Society - ISSN 1543-8384
Roč. 16, č. 8 (2019), s. 3441-3451Number of pages 11 s. Publication form Print - P Language eng - English Country US - United States Keywords fluorescent nanodiamonds ; cell immunopathology ; nanodiamond intercellular distribution ; inflammation ; inflammasome NLRP3 ; AFM ; TEM ; cathodoluminescence Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering OECD category Nano-processes (applications on nano-scale) Subject RIV - cooperation Institute of Physics - Biophysics R&D Projects EF16_019/0000760 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA17-15451S GA ČR - Czech Science Foundation (CSF) LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support UPT-D - RVO:68081731 ; FZU-D - RVO:68378271 UT WOS 000480371700011 EID SCOPUS 85068455612 DOI 10.1021/acs.molpharmaceut.9b00225 Annotation Nanodiamonds (ND), especially fluorescent NDs, represent potentially applicable drug and probe carriers for in vitro/in vivo applications. The main purpose of this study was to relate physical-chemical properties of carboxylated NDs to their intracellular distribution and impact on membranes and cell immunity-activation of inflammasome in the in vitro THP-1 cell line model. Dynamic light scattering, nanoparticle tracking analysis, and microscopic methods were used to characterize ND particles and their intracellular distribution. Fluorescent NDs penetrated the cell membranes by both macropinocytosis and mechanical cutting through cell membranes. We proved accumulation of fluorescent NDs in lysosomes. In this case, lysosomes were destabilized and cathepsin B was released into the cytoplasm and triggered pathways leading to activation of inflammasome NLRP3, as detected in THP-1 cells. Activation of inflammasome by NDs represents an important event that could underlie the described toxicological effects in vivo induced by NDs. According to our knowledge, this is the first in vitro study demonstrating direct activation of inflammasome by NDs. These findings are important for understanding the mechanism(s) of action of ND complexes and explain the ambiguity of the existing toxicological data. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2020 Electronic address https://pubs.acs.org/doi/10.1021/acs.molpharmaceut.9b00225
Number of the records: 1