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Biomolecular charges influence the response of surface plasmon resonance biosensors through electronic and ionic mechanisms
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SYSNO ASEP 0504701 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Biomolecular charges influence the response of surface plasmon resonance biosensors through electronic and ionic mechanisms Author(s) Šípová, Hana (URE-Y)
Jurgová, Ludmila (URE-Y)
Mrkvová, Kateřina (URE-Y)
Lynn, Nicholas Scott (URE-Y) RID
Špačková, Barbora (URE-Y)
Homola, Jiří (URE-Y) RIDNumber of authors 6 Source Title Biosensors and Bioelectronics. - : Elsevier - ISSN 0956-5663
Roč. 126, 1 February (2019), s. 365-372Number of pages 8 s. Publication form Print - P Language eng - English Country NL - Netherlands Keywords Self-assembled monolayers of alkanethiols ; Biomolecules ; Surface charge Subject RIV BH - Optics, Masers, Lasers OECD category Optics (including laser optics and quantum optics) R&D Projects GBP205/12/G118 GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support URE-Y - RVO:67985882 UT WOS 000457659500047 EID SCOPUS 85057082094 DOI 10.1016/j.bios.2018.11.002 Annotation Surface plasmon resonance (SPR) biosensors have become an important label-free optical biomolecular sensing technology and a 'gold standard' for retrieving information on the kinetics of biomolecular interactions. Even though biomolecules typically contain an abundance of easily ionizable chemical groups, there is a gap in understanding of whether (and how) the electrostatic charge of a biomolecular system influences the SPR biosensor response. In this work we show that negative static charge present in a biomolecular layer on the surface of an SPR sensor results in significant SPR spectral shifts, and we identify two major mechanisms responsible for such shifts: 1) the formation of an electrical double layer (ionic mechanism), and 2) changes in the electron density at the surface of a metal (electronic mechanism). We show that under low ionic strength conditions, the electronic mechanism is dominant and the SPR wavelength shift is linearly proportional to the surface concentration of biomolecular charges. At high ionic strength conditions, both electric and ionic mechanisms contribute to the SPR wavelength shift. Using the electronic mechanism, we estimated the pKa of surface-bound carboxylic groups and the relative concentration of the carboxyl-terminated alkanethiols in a binary self-assembled monolayer of alkanethiols. The reported sensitivity of SPR to surface charge is especially important in the context of biomolecular sensing. Moreover, it provides an avenue for the application of SPR sensors for fast, label-free determination of the net charge of a biomolecular coating, which is of interest in material science, surface chemistry, electrochemistry, and other fields. Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2020 Electronic address https://www.sciencedirect.com/science/article/pii/S0956566318308893?via%3Dihub
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