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Dose-rate effects in breaking DNA strands by short pulses of extreme ultraviolet radiation
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SYSNO ASEP 0503532 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Dose-rate effects in breaking DNA strands by short pulses of extreme ultraviolet radiation Author(s) Vyšín, L. (CZ)
Burian, Tomáš (UFP-V) ORCID
Ukrajintsev, E. (CZ)
Davídková, M. (CZ)
Grisham, M. E. (US)
Heinbuch, S. (US)
Rocca, J.J. (US)
Juha, Libor (UFP-V) ORCIDNumber of authors 8 Source Title Radiation Research. - : Radiation Research Society - ISSN 0033-7587
Roč. 189, č. 5 (2018), s. 466-476Number of pages 11 s. Language eng - English Country US - United States Keywords breaking DNA strands ; extreme ultraviolet radiation ; short pulses Subject RIV BH - Optics, Masers, Lasers OECD category Optics (including laser optics and quantum optics) Institutional support UFP-V - RVO:61389021 UT WOS 000430572800003 EID SCOPUS 85045934898 DOI 10.1667/RR14825.1 Annotation In this study, we examined dose-rate effects on strand break formation in plasmid DNA induced by pulsed extreme ultraviolet (XUV) radiation. Dose delivered to the target molecule was controlled by attenuating the incident photon flux using aluminum filters as well as by changing the DNA/buffer-salt ratio in the irradiated sample. Irradiated samples were examined using agarose gel electrophoresis. Yields of single- and double-strand breaks (SSBs and DSBs) were determined as a function of the incident photon fluence. In addition, electrophoresis also revealed DNA cross-linking. Damaged DNA was inspected by means of atomic force microscopy (AFM). Both SSB and DSB yields decreased with dose rate increase. Quantum yields of SSBs at the highest photon fluence were comparable to yields of DSBs found after synchrotron irradiation. The average SSB/DSB ratio decreased only slightly at elevated dose rates. In conclusion, complex and/or clustered damages other than cross-links do not appear to be induced under the radiation conditions applied in this study. Workplace Institute of Plasma Physics Contact Vladimíra Kebza, kebza@ipp.cas.cz, Tel.: 266 052 975 Year of Publishing 2019 Electronic address https://bioone.org/journals/Radiation-Research/volume-190/issue-2/RR00LV.1/Erratum/10.1667/RR00LV.1.short
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