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Evaluation of the 33 synthesized nitrophenol-based markers of isoelectic point

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    0498544 - ÚIACH 2019 RIV CZ eng C - Conference Paper (international conference)
    Duša, Filip - Moravcová, Dana - Šlais, Karel
    Evaluation of the 33 synthesized nitrophenol-based markers of isoelectic point.
    CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2018 - (Foret, F.; Křenková, J.; Drobníková, I.; Klepárník, K.; Přikryl, J.), s. 132-135. ISBN 978-80-904959-5-1.
    [CECE 2018. International Interdisciplinary Meeting on Bioanalysis /15./. Brno (CZ), 15.10.2018-17.10.2018]
    R&D Projects: GA ČR(CZ) GA16-03749S; GA MV VI20172020069
    Institutional support: RVO:68081715
    Keywords : nitrophenol-based markers * isoelectic point * capillary isoelectric focusing
    OECD category: Analytical chemistry
    http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf

    33 nitrophenol-based compounds, which absorb at ultraviolet as well as visible wavelengths, were selected as candidates for marking isoelectric points (pI) over the pH range from 3 to 10. The evaluation and selection of the candidate structures included structure-based calculations of acid-base properties. The selected compounds were further tested experimentally using capillary isoelectric focusing. The experimental analysis excluded two compounds due to producing double peaks during the analysis. Overall, 31 novel compounds were identified as suitable pI markers.
    Permanent Link: http://hdl.handle.net/11104/0290886

     
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