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Electrochemical sensing of interaction of anterior gradient-2 protein with peptides at a charged interface
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SYSNO ASEP 0492336 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Electrochemical sensing of interaction of anterior gradient-2 protein with peptides at a charged interface Author(s) Ostatná, Veronika (BFU-R) RID, ORCID
Kasalová, Veronika (BFU-R) ORCID
Sommerová, L. (CZ)
Hrstka, R. (CZ)Number of authors 4 Source Title Electrochimica acta. - : Elsevier - ISSN 0013-4686
Roč. 269, APR 10 2018 (2018), s. 70-75Number of pages 6 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords mercury-electrodes ; aptamers ; therapeutics ; evolution Subject RIV CG - Electrochemistry OECD category Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) R&D Projects GA15-21855S GA ČR - Czech Science Foundation (CSF) EF15_003/0000477 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BFU-R - RVO:68081707 UT WOS 000428806700009 DOI 10.1016/j.electacta.2018.02.152 Annotation Anterior gradient-2 protein (AGR2) is overexpressed in many human cancers, and this protein presents a novel promising cancer biomarker. We show significant progress in understanding of the electric field effects on AGR2-peptides complexes using constant current chronopotentiometric stripping (CPS) analysis. Surface-attached AGR2-peptide complexes can be disintegrated as a result of their exposure to negative potentials. By controlling the exposure time and temperature, peaks of weakly bound nonspecific complexes can be discriminated from tightly bound specific complexes. Using CPS analysis we found that mutant E60A-AGR2 forms weaker complex with peptide aptamer in comparison to wild type AGR2. These data highlight the utility of this method for studying real-time dynamics of surface-attached protein-peptide complexes. (C) 2018 Elsevier Ltd. All rights reserved. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2019
Number of the records: 1