Extensive flagellar remodeling during the complex life cycle of &ITParatrypanosoma&IT, an early-branching trypanosomatid

0485318 - BC 2018 RIV US eng J - Journal Article
Skalický, Tomáš - Dobáková, Eva - Wheeler, R. J. - Tesařová, Martina - Flegontov, P. - Jirsová, Dagmar - Votýpka, Jan - Yurchenko, V. - Ayala, F. J. - Lukeš, Julius
Extensive flagellar remodeling during the complex life cycle of &ITParatrypanosoma&IT, an early-branching trypanosomatid.
Proceedings of the National Academy of Sciences of the United States of America. Roč. 114, č. 44 (2017), s. 11757-11762. ISSN 0027-8424. E-ISSN 1091-6490
R&D Projects: GA ČR(CZ) GA14-23986S; GA MŠMT LL1601; GA MŠMT(CZ) LM2015062
Institutional support: RVO:60077344
Keywords : trypanosomatid * evolution * flagellar remodeling * haptomonads * cytostome
OECD category: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Impact factor: 9.504, year: 2017

Paratrypanosoma confusum is a monoxenous kinetoplastid flagellate that constitutes the most basal branch of the highly diverse parasitic trypanosomatids, which include human pathogens Trypanosoma and Leishmania. This makes Paratrypanosoma uniquely informative for the evolution of obligatory parasitism from free-living lifestyle and the evolution of human parasitism in some trypanosomatid lineages. It has typical promastigote morphology but also forms surface-attached haptomonads and amastigotes. Haptomonads form by attachment to a surface via a large bulge at the base of the flagellum, which is then remodeled into a thin attachment pad associated with flagellum shortening. Promastigotes and haptomonads multiply by binary division, and the progeny of a haptomonad can either remain attached or grow a flagellum and resume swimming. Whole genome sequencing and transcriptome profiling, in combination with analysis of the cell ultrastructure, reveal how the cell surface and metabolism are adapted to parasitism and how characteristic cytoskeletal features are conserved. Our data demonstrate that surface attachment by the flagellum and the flagellar pocket, a Leishmania-Wke flagellum attachment zone, and a Trypanosoma cruzi-like cytostome are ancestral features, while evolution of extant trypanosomatids, including the human parasites, is associated with genome streamlining and diversification of membrane proteins.
Permanent Link: http://hdl.handle.net/11104/0280385