The combination of nanofibrous and microfibrous materials for enhancement of cell infiltration and in vivo bone tissue formation

0482682 - ÚEM 2019 RIV GB eng J - Journal Article
Rampichová, Michala - Chvojka, J. - Jenčová, V. - Kubíková, T. - Tonar, Z. - Erben, J. - Buzgo, M. - Daňková, Jana - Litvinec, Andrej - Vocetková, Karolína - Plencner, Martin - Prosecká, Eva - Sovková, Věra - Lukášová, Věra - Králíčková, M. - Lukáš, D. - Amler, Evžen
The combination of nanofibrous and microfibrous materials for enhancement of cell infiltration and in vivo bone tissue formation.
Biomedical Materials. Roč. 13, č. 2 (2018), č. článku 025004. ISSN 1748-6041. E-ISSN 1748-605X
R&D Projects: GA ČR(CZ) GA15-15697S; GA MŠMT(CZ) LO1508; GA MŠMT(CZ) LO1309
Institutional support: RVO:68378041
Keywords : melt-blown * electrospinning * electroblowing * 3D fibers
OECD category: Orthopaedics
Impact factor: 3.440, year: 2018

Fibrous scaffolds are desired in tissue engineering applications for their ability to mimic extracellular matrix. In this study we compared fibrous scaffolds prepared from polycaprolactone using three different fabrication methods, electrospinning ES, electro-blowing and melt-blown combined with ES. Scaffolds differed in morphology, fiber diameters and pore sizes. Mesenchymal stem cell adhesion, proliferation and osteogenic differentiation on scaffolds was evaluated. The most promising scaffold was shown to be melt-blown in combination with ES which combined properties of both technologies. Microfibers enabled good cell infiltration and nanofibers enhanced cell adhesion. This scaffold was used for further testing in critical sized defects in rabbits. New bone tissue formation occurred from the side of the treated defects, compared to a control group where only fat tissue was present. Polycaprolactone fibrous scaffold prepared using a combination of melt-blown and ES technology seems to be promising for bone regeneration. The practical application of results is connected with enormous production capacity and low cost of materials produced by melt-blown technology, compared to other bone scaffold fabrication methods.
Permanent Link: http://hdl.handle.net/11104/0285962