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Nitro-oleic acid regulates growth factor-induced differentiation of bone marrow-derived macrophages
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SYSNO ASEP 0476647 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Nitro-oleic acid regulates growth factor-induced differentiation of bone marrow-derived macrophages Author(s) Vereščáková, Hana (BFU-R) ORCID
Ambrožová, Gabriela (BFU-R) RID
Kubala, Lukáš (BFU-R) RID, ORCID
Perečko, Tomáš (BFU-R) RID, ORCID
Koudelka, Adolf (BFU-R) ORCID
Vašíček, Ondřej (BFU-R) ORCID, RID
Rudolph, T.K. (DE)
Klinke, A. (DE)
Woodcock, S.R. (US)
Freeman, B.A. (US)
Pekarová, Michaela (BFU-R) RIDNumber of authors 11 Source Title Free Radical Biology and Medicine. - : Elsevier - ISSN 0891-5849
Roč. 104, MAR2017 (2017), s. 10-19Number of pages 10 s. Publication form Print - P Language eng - English Country US - United States Keywords colony-stimulating factor ; nitrated fatty-acids ; hematopoietic stem-cells ; gm-csf Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects GP13-40824P GA ČR - Czech Science Foundation (CSF) GJ17-08066Y GA ČR - Czech Science Foundation (CSF) LD15069 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BFU-R - RVO:68081707 UT WOS 000395968300002 DOI 10.1016/j.freeradbiomed.2017.01.003 Annotation Many diseases accompanied by chronic inflammation are connected with dysregulated activation of macrophage subpopulations. Recently, we reported that nitro-fatty acids (NO2-FAs), products of metabolic and inflammatory reactions of nitric oxide and nitrite, modulate macrophage and other immune cell functions. Bone marrow cell suspensions were isolated from mice and supplemented with macrophage colony-stimulating factor (M-CSF) or granulocyte-macrophage colony-stimulating factor (GM-CSF) in combination with NO2-OA for different times. RAW 264.7 macrophages were used for short-term (1-5 min) experiments. We discovered that NO2-OA reduces cell numbers, cell colony formation, and proliferation of macrophages differentiated with colony-stimulating factors (CSFs), all in the absence of toxicity. In a case of GM-CSF-induced bone marrow-derived macrophages (BMMs), NO2-OA acts via downregulation of signal transducer and activator of transcription 5 and extracellular signal-regulated kinase (ERK) activation. In the case of M-CSF-induced BMMs, NO2-OA decreases activation of M-CSFR and activation of related PI3K and ERR. Additionally, NO2-OA also attenuates activation of BMMs. In aggregate, we demonstrate that NO2-OA regulates the process of macrophage differentiation and that NO2-FAs represent a promising therapeutic tool in the treatment of inflammatory pathologies linked with increased accumulation of macrophages in inflamed tissues. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2018
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