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Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondria! DNA-deficient cancer cells
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SYSNO ASEP 0474268 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondria! DNA-deficient cancer cells Author(s) Dong, L. F. (AU)
Kovářová, Jaromíra (BTO-N) RID
Bajziková, Martina (BTO-N) RID
Bezawork-Geleta, A. (AU)
Švec, David (BTO-N) RID
Endaya, B. (AU)
Sachaphibulkij, K. (AU)
Coelho, Ana R. (BTO-N)
Šebková, Nataša (BTO-N)
Ruzickova, A. (CZ)
Tan, A.S. (NZ)
Klučková, Katarína (BTO-N) RID
Judasová, Kateřina (BTO-N)
Zamecnikova, K. (CZ)
Rychtarčíková, Zuzana (BTO-N)
Gopalan, V. (US)
Anděra, Ladislav (BTO-N)
Sobol, Margaryta (UMG-J) RID
Yan, B. (AU)
Pattnaik, B. (IN)
Bhatraju, N. (IN)
Truksa, Jaroslav (BTO-N) RID, ORCID
Stopka, P. (CZ)
Hozák, Pavel (UMG-J) RID, ORCID
Lam, A. K. (AU)
Sedláček, Radislav (UMG-J) RID
Oliveira, P.J. (PT)
Kubista, Mikael (BTO-N) RID
Agrawal, A. (IN)
Dvořáková-Hortová, Kateřina (BTO-N)
Rohlena, Jakub (BTO-N) RID, ORCID
Berridge, M.V. (NZ)
Neužil, Jiří (BTO-N) RIDArticle number e22187 Source Title eLife. - : eLife - ISSN 2050-084X
Roč. 6, 15 Febr 2017 (2017)Number of pages 22 s. Language eng - English Country US - United States Keywords SUPPORTING ASPARTATE BIOSYNTHESIS ; ELECTRON-TRANSPORT CHAIN ; OXIDATIVE-PHOSPHORYLATION Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology Subject RIV - cooperation Institute of Molecular Genetics - Genetics ; Molecular Biology R&D Projects GA16-12816S GA ČR - Czech Science Foundation (CSF) LM2011032 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA16-22823S GA ČR - Czech Science Foundation (CSF) GA17-20904S GA ČR - Czech Science Foundation (CSF) LO1419 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA16-12719S GA ČR - Czech Science Foundation (CSF) GA15-02203S GA ČR - Czech Science Foundation (CSF) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BTO-N - RVO:86652036 ; UMG-J - RVO:68378050 UT WOS 000397644600001 EID SCOPUS 85016312406 DOI 10.7554/eLife.22187 Annotation Recently, we showed that generation of tumours in syngeneic mice by cells devoid of mitochondrial (mt) DNA (rho(0) cells) is linked to the acquisition of the host mtDNA. However, the mechanism of mtDNA movement between cells remains unresolved. To determine whether the transfer of mtDNA involves whole mitochondria, we injected B16 rho(0) mouse melanoma cells into syngeneic C576116N(su9DsRed2) mice that express red fluorescent protein in their mitochondria. We document that mtDNA is acquired by transfer of whole mitochondria from the host animal, leading to normalisation of mitochondrial respiration. Additionally, knockdown of key mitochondrial complex I (NDUFV1) and complex II (SDHC) subunits by shRNA in B16 rho(0) cells abolished or significantly retarded their ability to form tumours. Collectively, these results show that intact mitochondria with their mtDNA payload are transferred in the developing tumour, and provide functional evidence for an essential role of oxidative phosphorylation in cancer. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2018
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