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Hydrogen Evolution Facilitates Reduction of DNA Guanine Residues at the Hanging Mercury Drop Electrode: Evidence for a Chemical Mechanism
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SYSNO ASEP 0471945 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Hydrogen Evolution Facilitates Reduction of DNA Guanine Residues at the Hanging Mercury Drop Electrode: Evidence for a Chemical Mechanism Author(s) Daňhel, Aleš (BFU-R) RID, ORCID
Havran, Luděk (BFU-R) RID, ORCID
Trnková, L. (CZ)
Fojta, Miroslav (BFU-R) RID, ORCIDNumber of authors 4 Source Title Electroanalysis. - : Wiley - ISSN 1040-0397
Roč. 28, č. 11 (2016), s. 2785-2790Number of pages 6 s. Publication form Print - P Language eng - English Country DE - Germany Keywords central trinucleotide sequences ; cyclic voltammetry ; cisplatin Subject RIV BO - Biophysics R&D Projects GAP206/11/1638 GA ČR - Czech Science Foundation (CSF) GA16-01625S GA ČR - Czech Science Foundation (CSF) Institutional support BFU-R - RVO:68081707 UT WOS 000387891400020 DOI 10.1002/elan.201600242 Annotation Guanine (G), as well as G residues in nucleosides, nucleotides and nucleic acids, undergo chemically reversible (but electrochemically irreversible) reduction/oxidation processes at the mercury-based electrodes. It has been established that G is reduced to 7,8-dihydroguanine at highly negative potentials. The reduction product is oxidized back to G around0.25V, giving rise to anodic peak G. Previous studies suggested involvement of a chemical mechanism involving electrochemically generated hydrogen radicals in the G reduction process. In this work we studied effects of cisplatin and pH on the G reduction process. We have found that catalytic hydrogen evolution accompanying electrochemical reduction of cisplatin markedly facilitates reduction of G. Minimum negative potential required for G reduction were shifted to less negative values and correlated with the onset of catalytic currents of cisplatin. Analogous shifts of the potential of G reduction were observed upon lowering pH of the background electrolyte (i.e., increasing the availability of protons to generate hydrogen radicals). Ammonium ions markedly increased efficiency of G reduction, which may be explained by generation of active hydrogen via formation and subsequent decomposition of ammonium amalgam. Our results strongly suggest that chemical mechanism(s) involving hydrogen radicals, electrochemically and/or electrocatalytically generated at the HMDE, contribute to the guanine 7,8-dihydroguanine conversion. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2017
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