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Post-transcriptional mending of gene sequences: looking under the hood of mitochondrial gene expression in diplonemids
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SYSNO ASEP 0468794 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Post-transcriptional mending of gene sequences: looking under the hood of mitochondrial gene expression in diplonemids Author(s) Valach, M. (CA)
Moreira, S. (CA)
Faktorová, Drahomíra (BC-A) RID, ORCID
Lukeš, Julius (BC-A) RID, ORCID
Burger, G. (CA)Source Title RNA biology. - : Taylor & Francis - ISSN 1547-6286
Roč. 13, č. 12 (2016), s. 1204-1211Number of pages 8 s. Publication form Print - P Language eng - English Country US - United States Keywords Cryptic genes ; gene fragmentation ; trans-splicing ; RNA editing ; multipartite mtDNA ; diplonemids Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA15-21974S GA ČR - Czech Science Foundation (CSF) Institutional support BC-A - RVO:60077344 UT WOS 000390670300005 EID SCOPUS 85003794187 DOI 10.1080/15476286.2016.1240143 Annotation The instructions to make proteins and structural RNAs are laid down in gene sequences. Yet, in certain instances, these primary instructions need to be modified considerably during gene expression, most often at the transcript level. Here we review a case of massive post-transcriptional revisions via trans-splicing and RNA editing, a phenomenon occurring in mitochondria of a recently recognized protist group, the diplonemids. As of now, the various post-transcriptional steps have been cataloged in detail, but how these processes function is still unknown. Since genetic manipulation techniques such as gene replacement and RNA interference have not yet been established for these organisms, alternative strategies have to be deployed. Here, we discuss the experimental and bioinformatics approaches that promise to unravel the molecular machineries of trans-splicing and RNA editing in Diplonema mitochondria. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2017
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