RGDS- and SIKVAVS-modified superporous poly(2-hydroxyethyl methacrylate) scaffolds for tissue engineering applications

0465604 - ÚMCH 2017 RIV DE eng J - Journal Article
Macková, Hana - Plichta, Zdeněk - Proks, Vladimír - Kotelnikov, Ilya - Kučka, Jan - Hlídková, Helena - Horák, Daniel - Kubinová, Šárka - Jiráková, Klára
RGDS- and SIKVAVS-modified superporous poly(2-hydroxyethyl methacrylate) scaffolds for tissue engineering applications.
Macromolecular Bioscience. Roč. 16, č. 11 (2016), s. 1621-1631. ISSN 1616-5187. E-ISSN 1616-5195
R&D Projects: GA ČR(CZ) GA14-14961S; GA ČR(CZ) GA16-02702S; GA MŠMT(CZ) LO1309; GA MŠMT(CZ) LQ1604; GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT(CZ) 7F14057
Institutional support: RVO:61389013 ; RVO:68378041
Keywords : polymer drug carriers * tumor targeting * enzymatic release
Subject RIV: CD - Macromolecular Chemistry; FH - Neurology (UEM-P)
Impact factor: 3.238, year: 2016

Three-dimensional hydrogel supports for mesenchymal and neural stem cells (NSCs) are promising materials for tissue engineering applications such as spinal cord repair. This study involves the preparation and characterization of superporous scaffolds based on a copolymer of 2-hydroxyethyl and 2-aminoethyl methacrylate (HEMA and AEMA) crosslinked with ethylene dimethacrylate. Ammonium oxalate is chosen as a suitable porogen because it consists of needle-like crystals, allowing their parallel arrangement in the polymerization mold. The amino group of AEMA is used to immobilize RGDS and SIKVAVS peptide sequences with an N-gamma-maleimidobutyryloxy succinimide ester linker. The amount of the peptide on the scaffold is determined using 125I radiolabeled SIKVAVS. Both RGDS- and SIKVAVS-modified poly(2-hydroxyethyl methacrylate) scaffolds serve as supports for culturing human mesenchymal stem cells (MSCs) and human fetal NSCs. The RGDS sequence is found to be better for MSC and NSC proliferation and growth than SIKVAVS.
Permanent Link: http://hdl.handle.net/11104/0264244