Number of the records: 1
The essential function of the Trypanosoma brucei Trl1 homolog in procyclic cells is maturation of the intron-containing tRNATyr
- 1.
SYSNO ASEP 0463449 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The essential function of the Trypanosoma brucei Trl1 homolog in procyclic cells is maturation of the intron-containing tRNATyr Author(s) Lopes, R.R.S. (BR)
Silveira, G. de O. (BR)
Eitler, R. (BR)
Vidal, R.S. (BR)
Kessler, A. (US)
Hinger, S. (US)
Paris, Zdeněk (BC-A) RID
Alfonzo, J. D. (US)
Polycarpo, C. (BR)Source Title RNA. - : Cold Spring Harbor Laboratory Press - ISSN 1355-8382
Roč. 22, č. 8 (2016), s. 1190-1199Number of pages 10 s. Publication form Print - P Language eng - English Country US - United States Keywords Trypanosoma ; tRNA ; tRNA editing ; splicing ; intron Subject RIV EB - Genetics ; Molecular Biology R&D Projects GJ15-21450Y GA ČR - Czech Science Foundation (CSF) Institutional support BC-A - RVO:60077344 UT WOS 000386679200009 EID SCOPUS 84979686900 DOI 10.1261/rna.056242.116 Annotation Trypanosoma brucei, the etiologic agent of sleeping sickness, encodes a single intron-containing tRNA, tRNA(Tyr), and splicing is essential for its viability. In Archaea and Eukarya, tRNA splicing requires a series of enzymatic steps that begin with intron cleavage by a tRNA-splicing endonuclease and culminates with joining the resulting tRNA exons by a splicing tRNA ligase. Here we explored the function of TbTrl1, the T. brucei homolog of the yeast Trl1 tRNA ligase. We used a combination of RNA interference and molecular biology approaches to show that down-regulation of TbTrl1 expression leads to accumulation of intron-containing tRNA(Tyr) and a concomitant growth arrest at the G1 phase. These defects were efficiently rescued by expression of an "intronless" version of tRNA(Tyr) in the same RNAi cell line. Taken together, these experiments highlight the crucial importance of the TbTrl1 for tRNA(Tyr) maturation and viability, while revealing tRNA splicing as its only essential function Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2017
Number of the records: 1