A calcium-accumulating region, CAR, in the channel Orai1 enhances Ca2+ permeation and SOCE-induced gene transcription

0463207 - MBÚ 2017 RIV US eng J - Journal Article
Frischauf, I. - Zayats, Vasilina - Deix, M. - Hochreiter, A. - Jardin, I. - Muik, M. - Lackner, B. - Svobodová, B. - Pammer, T. - Litviňuková, M. - Sridhar, A.A. - Derler, I. - Bogeski, I. - Romanin, Ch. - Ettrich, Rüdiger - Schindel, R.
A calcium-accumulating region, CAR, in the channel Orai1 enhances Ca2+ permeation and SOCE-induced gene transcription.
Science Signaling. Roč. 8, č. 408 (2015), ra131. ISSN 1945-0877. E-ISSN 1937-9145
R&D Projects: GA ČR GA13-21053S
Institutional support: RVO:61388971
Keywords : MOLECULAR-DYNAMICS SIMULATIONS * CRAC CHANNEL * PORE ARCHITECTURE
Subject RIV: EE - Microbiology, Virology
Impact factor: 7.359, year: 2015

The Ca2+ release-activated Ca2+ channel mediates Ca2+ influx in a plethora of cell types, thereby controlling diverse cellular functions. The channel complex is composed of stromal interaction molecule 1 (STIM1), an endoplasmic reticulum Ca2+-sensing protein, and Orai1, a plasma membrane Ca2+ channel. Channels composed of STIM1 and Orai1 mediate Ca2+ influx even at low extracellular Ca2+ concentrations. We investigated whether the activity of Orai1 adapted to different environmental Ca2+ concentrations. We used homology modeling and molecular dynamics simulations to predict the presence of an extracellular Ca2+-accumulating region (CAR) at the pore entrance of Orai1.
Permanent Link: http://hdl.handle.net/11104/0262450