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CD46 and β1 integrin relocation in the sperm head during capacitaion and acrosome reaction
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SYSNO ASEP 0463176 Document Type C - Proceedings Paper (int. conf.) R&D Document Type The record was not marked in the RIV Title CD46 and β1 integrin relocation in the sperm head during capacitaion and acrosome reaction Author(s) Šebková, Nataša (BTO-N)
Frolíková, Michaela (BTO-N) ORCID
Děd, Lukáš (BTO-N) RID
Dvořáková-Hortová, Kateřina (BTO-N)Number of authors 4 Source Title Abstract book of the 48th Annual Meeting of the Society for the Study of Reproduction. - San Juan : Society for the Study of Reproduction, 2015 Action 48th Annual Meeting of the Society for the Study of reproduction Event date 18.06.2015-22.06.2015 VEvent location San Juan Country US - United States Event type WRD Language eng - English Country US - United States Keywords CD46 ; β1 integrin ; acrosome reaction ; capacitation R&D Projects GA14-05547S GA ČR - Czech Science Foundation (CSF) Annotation CD46 protein plays an important role during fertilization and its role is associated with acrosome stability. CD46 is probably involved in signaling pathways triggering the acrosome reaction (AR). It also associates, through membrane integrins, with specific MAP kinases involved in AR. Our aim was to monitor the possible dynamics of relocation and movement of CD46 and β1 integrin during sperm maturation and its preparation for the fertilization. Our results show changes in the localization of these proteins associated with the AR and their mutual co-localization was observed. The β1integrin location in the freshly released epididymal sperm is in the acrosome and it relocates during the AR further through the sperm head compartments into the equatorial segment and over the whole sperm head. Its density over the equatorial segment is decreasing with the extended time of the capacitation. Also its presence in the perforatorium of the mouse sperm head is very prominent. The pattern for CD46 is extremely similar if not identical in both aspects such as compartment localization and time progress during capacitation and AR in vitro. Similarly to CD46, β1integrin was observed progressing across the apical acrosome and the equatorial segment. However, its relocation carries on further to the postacrosome region of the sperm head. The molecular interaction of CD46 and β1integrin is being investigated using the Proximity Ligation Assay. The data were statistically analysed. In summary, our results deliver new information that proteins CD46 and β1 integrin undergo dynamic relocation towards the sites of sperm-egg fusion during the AR in vitro. We speculate that this relocation is of importance for the successful sperm-egg interaction, adhesion and subsequent gamete fusion. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2017
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