Bordetella pertussis filamentous hemagglutinin itself does not trigger anti-inflammatory interleukin-10 production by human dendritic cells

0462464 - MBÚ 2017 RIV DE eng J - Journal Article
Romero, Rodrigo, Villarino - Hasan, Shakir - Faé, K. - Holubová, Jana - Geurtsen, J. - Schwarzer, Martin - Wiertsema, S. - Osička, Radim - Poolman, J. - Šebo, Peter
Bordetella pertussis filamentous hemagglutinin itself does not trigger anti-inflammatory interleukin-10 production by human dendritic cells.
International Journal of Medical Microbiology. Roč. 306, č. 1 (2016), s. 38-47. ISSN 1438-4221. E-ISSN 1618-0607
R&D Projects: GA ČR GA15-09157S; GA ČR GA13-14547S
Institutional support: RVO:61388971
Keywords : Bordetella pertussis * Cytokine * Dendritic cell
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.391, year: 2016

Filamentous hemagglutinin (FHA) is an important adhesin of the whooping cough agent Bordetella pertussis and is contained in most acellular pertussis vaccines. Recently, FHA was proposed to exert an immunomodulatory activity through induction of tolerogenic IL-10 secretion from dendritic cells. We have re-evaluated the cytokine-inducing activity of FHA, placing specific emphasis on the role of the residual endotoxin contamination of FHA preparations. We show that endotoxin depletion did not affect the capacity of FHA to bind primary human monocyte-derived dendritic cells, while it abrogated the capacity of FHA to elicit TNF-alpha and IL-10 secretion and strongly reduced its capacity to trigger IL-6 production.
Permanent Link: http://hdl.handle.net/11104/0261932