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Label-free electrochemical detection of singlet oxygen protein damage
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SYSNO ASEP 0456295 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Label-free electrochemical detection of singlet oxygen protein damage Author(s) Vargová, Veronika (BFU-R)
Gimenez, R.E. (AR)
Černocká, Hana (BFU-R) RID, ORCID
Trujillo, D.C. (AR)
Tulli, F. (AR)
Zanini, V.I.P. (AR)
Paleček, Emil (BFU-R) RID, ORCID
Borsarelli, C.D. (AR)
Ostatná, Veronika (BFU-R) RID, ORCIDNumber of authors 10 Source Title Electrochimica acta. - : Elsevier - ISSN 0013-4686
Roč. 187, JAN 2016 (2016), s. 662-669Number of pages 8 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords singlet oxygen protein damage ; surface-attached protein stability ; mercury and carbon electrodes Subject RIV BO - Biophysics R&D Projects GA13-00956S GA ČR - Czech Science Foundation (CSF) Institutional support BFU-R - RVO:68081707 UT WOS 000367235600077 DOI 10.1016/j.electacta.2015.11.104 Annotation Oxidative damage of proteins results in changes of their structures and functions. In this work, the singlet oxygen (O-1(2))-mediated oxidation of bovine serum albumin (BSA) and urease by blue-light photosensitization of the tris(2,2'-bipyridine)ruthenium(II) cation [Ru(bpy)(3)](2+) was studied by square wave voltammetry at glassy carbon electrode and by constant current chronopotentiometry at mercury electrode. Small changes in voltammetric oxidation Tyr and Trp peaks did not indicate significant changes in the BSA structure after photo-oxidation at carbon electrode. On the other hand chronopotentiometric peak H of BSA at HMDE increased during blue-light photosensitization, indicating that photo-oxidized BSA was more susceptible to the electric field-induced denaturation than non-oxidized native BSA. Similar results were obtained for urease, where enzymatic activity was also evaluated. The present results show the capability of label- and reagent-free electrochemical methods to detect oxidative changes in proteins. We believe that these methods will become important tools for detection of various protein damages. (C) 2015 Elsevier Ltd. All rights reserved. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2016
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