Biophysical properties of cationic lipophosphoramidates: Vesicle morphology, bilayer hydration and dynamics

Loizeau, D.; Jurkiewicz, Piotr; Aydogan, Gokcan; Philimonenko, Anatoly; Mahfoudhi, S.; Hozák, Pavel; Maroto, A.; Couthon-Courves, H.; Jaffres, P.-A.; Deschamps, L.; Giamarchi, P.; Hof, Martin

doi:https://dx.doi.org/10.1016/j.colsurfb.2015.09.012

Number of the records: 1

Biophysical properties of cationic lipophosphoramidates: Vesicle morphology, bilayer hydration and dynamics

1.

SYSNO ASEP	0447841
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Biophysical properties of cationic lipophosphoramidates: Vesicle morphology, bilayer hydration and dynamics
Author(s)	Loizeau, D. (FR) Jurkiewicz, Piotr (UFCH-W)_{RID, ORCID} Aydogan, Gokcan (UFCH-W) Philimonenko, Anatoly (UMG-J) Mahfoudhi, S. (FR) Hozák, Pavel (UMG-J)_{RID, ORCID} Maroto, A. (FR) Couthon-Courves, H. (FR) Jaffres, P.-A. (FR) Deschamps, L. (FR) Giamarchi, P. (FR) Hof, Martin (UFCH-W)_{RID, ORCID}
Source Title	Colloids and Surfaces B-Biointerfaces. - : Elsevier - ISSN 0927-7765 Roč. 136, DEC 2015 (2015), s. 192-200
Number of pages	9 s.
Language	eng - English
Country	NL - Netherlands
Keywords	gene therapy ; transfection ; non-viral DNA vector
Subject RIV	CF - Physical ; Theoretical Chemistry
Subject RIV - cooperation	Institute of Molecular Genetics - Genetics ; Molecular Biology
R&D Projects	GBP208/12/G016 GA ČR - Czech Science Foundation (CSF)
Institutional support	UFCH-W - RVO:61388955 ; UMG-J - RVO:68378050
UT WOS	000367408100025
EID SCOPUS	84941945136
DOI	https://doi.org/10.1016/j.colsurfb.2015.09.012
Annotation	Cationic lipids are used to deliver genetic material to living cells. Their proper biophysical characterization is needed in order to design and control this process. In the present work we characterize some properties of recently synthetized cationic lipophosphoramidates. The studied compounds share the same structure of their hydrophobic backbone, but differ in their hydrophilic cationic headgroup, which is formed by a trimethylammonium, a trimethylarsonium or a dicationic moiety. Dynamic light scattering and cryo-transmission electron microscopy proves that the studied lipophosphoramidates create stable unilamellar vesicles. Fluorescence of polarity probe, Laurdan, analyzed using time-dependent fluorescence shift method (TDFS) and generalized polarization (GP) gives important information about the phase, hydration and dynamics of the lipophosphoramidate bilayers. While all of the compounds produced lipid bilayers that were sufficiently fluid for their potential application in gene therapy, their polarity/hydration and mobility was lower than for the standard cationic lipid – DOTAP. Mixing cationic lipophosphoramidates with DOPC helps to reduce this difference. The structure of the cationic headgroup has an important and complex influence on bilayer hydration and mobility. Both TDFS and GP methods are suitable for the characterization of cationic amphiphiles and can be used for screening of the newly synthesized compounds.
Workplace	J. Heyrovsky Institute of Physical Chemistry
Contact	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Year of Publishing	2016

Number of the records: 1