The effect of dibenzo-p-dioxin- and dibenzofuran-contaminated soil on the earthworm Eisenia andrei

0435255 - MBÚ 2015 RIV GB eng J - Journal Article
Roubalová, Radka - Dvořák, Jiří - Procházková, Petra - Elhottová, Dana - Rossmann, Pavel - Škanta, František - Bilej, Martin
The effect of dibenzo-p-dioxin- and dibenzofuran-contaminated soil on the earthworm Eisenia andrei.
Environmental Pollution. Roč. 193, OCT 2014 (2014), s. 22-28. ISSN 0269-7491. E-ISSN 1873-6424
R&D Projects: GA MŠMT(CZ) EE2.3.20.0055; GA MŠMT(CZ) EE2.3.30.0003; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:61388971 ; RVO:60077344
Keywords : Eisenia andrei * PCDD/Fs * Calreticulin
Subject RIV: EE - Microbiology, Virology
Impact factor: 4.143, year: 2014

Polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) belong to the group of persistent organic pollutants, highly toxic environmental pollutants that include hydrophobic compounds with the tendency to bioaccumulate. Earthworms (Eisenia andrei) were exposed to PCDD/Fs-contaminated soil, and changes in their lipophilic structures and the gene expression of their defense molecules were followed. Damage to the intestinal wall and adjacent chloragogenous tissue was observed. Further, the up-regulation of the expression of several genes was detected. On the basis of these results, the mechanism of the impact of PCDD/Fs on earthworms has been proposed. Dioxins that accumulate in the lipophilic structures cause an increase in reactive oxidative species that triggers oxidative stress followed by the gene expression of two molecules that play a role in protection against oxidant toxicity, calreticulin (CRT) and Hsp70. Moreover, the effect of microbial biomass on the expression of coelomic cytolytic factor (CCF), a pattern recognition receptor, was also observed.
Permanent Link: http://hdl.handle.net/11104/0239202