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Monitoring the influence of antibiotic exposure using Raman spectroscopy
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SYSNO ASEP 0434960 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Monitoring the influence of antibiotic exposure using Raman spectroscopy Author(s) Samek, Ota (UPT-D) RID, ORCID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCID
Bernatová, Silvie (UPT-D) RID, SAI
Ježek, Jan (UPT-D) RID, ORCID, SAI
Šerý, Mojmír (UPT-D) RID, SAI
Jákl, Petr (UPT-D) RID, ORCID, SAI
Šiler, Martin (UPT-D) RID, ORCID, SAI
Růžička, F. (CZ)Number of authors 8 Source Title Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry (Proceedings of SPIE 8939). - Bellingham : SPIE, 2014 - ISSN 0277-786X - ISBN 9780819498526 Pages 89390p-1-6 Number of pages 6 s. Publication form Print - P Action Biomedical Vibrational Spectroscopy /6./: Advances in Research and Industry Event date 01.02.2014-02.02.2014 VEvent location San Francisco Country US - United States Event type WRD Language eng - English Country US - United States Keywords Raman spectroscopy ; Fingerprint recognition ; Principal component analysis Subject RIV BH - Optics, Masers, Lasers R&D Projects LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GAP205/11/1687 GA ČR - Czech Science Foundation (CSF) Institutional support UPT-D - RVO:68081731 UT WOS 000334098200015 EID SCOPUS 84897466491 DOI 10.1117/12.2038595 Annotation Here we report on combination of the data obtained from MICs (minimum inhibitory concentrations) with infor- mation of microoragnisms fingerprint provided by Raman spectroscopy. In our feasibility study we could follow mechanisms of the bacteriostatic versus bactericidal action on biofilm-positive Staphylococcus epidermidis simply by monitoring Raman bands corresponding to DNA translating the changes introduced by selected antibiotics. The Raman spectra of Staphylococcus epidermidis treated with a bacteriostatic agent show little effect on DNA which is in contrast with the action of a bactericidal agent where decreased in dedicated Raman spectra signal strength suggests DNA fragmentation. Moreover, we demonstrate that Raman tweezers are indeed able to distinguish strains of biofilm-forming (biofilm-positive) and biofilm-negative Staphylococcus epidermidis strains using principal component analysis (PCA). Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2015
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