The isolation and characterization of lipopolysaccharides from Microcystis aeruginosa, a prominent toxic water bloom forming cyanobacteria

Blahová, L.; Adamovský, O.; Kubala, Lukáš; Šindlerová, Lenka; Zounková, R.; Blaha, L.

doi:https://dx.doi.org/10.1016/j.toxicon.2013.10.011

Number of the records: 1

The isolation and characterization of lipopolysaccharides from Microcystis aeruginosa, a prominent toxic water bloom forming cyanobacteria

1.

SYSNO ASEP	0424726
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The isolation and characterization of lipopolysaccharides from Microcystis aeruginosa, a prominent toxic water bloom forming cyanobacteria
Author(s)	Blahová, L. (CZ) Adamovský, O. (CZ) Kubala, Lukáš (BFU-R)_{RID, ORCID} Šindlerová, Lenka (BFU-R)_{RID, ORCID} Zounková, R. (CZ) Blaha, L. (CZ)
Number of authors	6
Source Title	Toxicon. - : Elsevier - ISSN 0041-0101 Roč. 76, DEC (2013), s. 187-196
Number of pages	10 s.
Publication form	Print - P
Language	eng - English
Country	GB - United Kingdom
Keywords	CYLINDROSPERMOPSIS-RACIBORSKII ; ESCHERICHIA-COLI ; DANIO-RERIO
Subject RIV	BO - Biophysics
Institutional support	BFU-R - RVO:68081707
UT WOS	000328658600023
EID SCOPUS	84886285044
DOI	10.1016/j.toxicon.2013.10.011
Annotation	Massive toxic blooms of cyanobacteria represent a major threat to water supplies worldwide, yet serious gaps exist in understanding their complex toxic effects, including the role of lipopolysaccharides (LPS). The present comparative study focused on the levels and biological activities of LPS isolated from Microcystis aeruginosa, which is one of the most globally distributed toxic species. Using hot phenol extraction, LPS was isolated from 3 laboratory cultures and 11 natural water blooms. It formed 0.2-0.7% of the original dry biomass of the cyanobacteria, based on gravimetry. Additional analyses by commercial anti-LPS ELISA were correlated with gravimetry but showed concentrations that were about 7-times lower, which indicated either impurities in isolated LPS or the poor cross-reactivity of the antibodies used. LPS isolates from M. aeruginosa were potent pyrogens in the traditional Limulus amebocyte lysate (LAL)-test, but comparison with the PyroGene test demonstrated the limited selectivity of LAL with several interferences.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2014

Number of the records: 1