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Overexpression of γ-tubulin in non-small cell lung cancer
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SYSNO ASEP 0387840 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Overexpression of γ-tubulin in non-small cell lung cancer Author(s) Maounis, N.F. (US)
Dráberová, Eduarda (UMG-J) RID, ORCID
Mahera, E. (GR)
Chorti, M. (GR)
Caracciolo, V. (US)
Sulimenko, Tetyana (UMG-J)
Riga, D. (GR)
Trakas, N. (GR)
Emmanouilidou, A. (GR)
Giordano, A. (US)
Dráber, Pavel (UMG-J) RID, ORCID
Katsetos, C.D. (US)Source Title Histology and Histopathology. - : Universidad de Murcia - ISSN 0213-3911
Roč. 27, č. 9 (2012), s. 1183-1194Number of pages 12 s. Language eng - English Country ES - Spain Keywords gamma-tubulin ; microtubules ; NSCLC Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA204/09/1777 GA ČR - Czech Science Foundation (CSF) GAP302/10/1701 GA ČR - Czech Science Foundation (CSF) LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000306521400007 Annotation WWe and others have previously shown that increased expression and altered compartmentalization of γ-tubulin may contribute to tumorigenesis and tumor progression (J. Cell Physiol. 2009;223:519-529; Cancer Biol. Ther. 2010;9:66-76). Here we have determined by immunohistochemistry the localization and cellular distribution of γ-tubulin in clinical tissue samples from 109 non-small cell lung cancer (NSCLC) cases. The expression and distribution of γ-tubulin protein and transcripts was also determined in the NSCLC tumor cell lines NCI-H460 (HTB-177) and NCI-H69 (HTB-119) by immunocytochemistry, quantitative immunoblotting and reverse transcription quantitative real-time PCR (RT-qPCR). Polyclonal and monoclonal anti-peptide antibodies recognizing epitopes in the C- or N-terminal domains of γ-tubulins and human gene-specific primers for γ-tubulins 1 (TUBG1) and 2 (TUBG2) were used. In non-neoplastic cells of the airway epithelium in situ, γ-tubulin exhibited predominantly apical surface and pericentriolar localizations. In contrast, markedly increased, albeit heterogeneous and variously prominent γ-tubulin immunoreactivity was detected in clinical tumor specimens and in the NCI-H460 and NCI-H69 cell lines, where tumor cells exhibited overlapping multi-punctate and diffuse patterns of localization. Co-expression of γ-tubulin and Ki-67 (MIB-1) was detected in a population of proliferating tumor cells. A statistically significant increase of γ-tubulin expression was found in Stage III compared to lesser stage tumors (p<0.001 v. Stages I/II) regardless of histological subtype or grade. By quantitative immunoblotting NCI-H460 and NCI-H69 cells expressed higher levels of γ-tubulin protein compared to small airway epithelial cells (SAEC). In both tumor cell lines increase in TUBG1 and TUBG2 transcripts was detected by RT-qPCR. Our results reveal for the first time an increased expression of γ-tubulin in lung cancer. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2013
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