Inhibition of proteasomal proteolysis affects expression of extracellular matrix components and steroidogenesis in porcine oocyte-cumulus complexes

0371581 - ÚŽFG 2012 RIV US eng J - Journal Article
Nagyová, Eva - Scsuková, S. - Němcová, Lucie - Mlynarčíková, A. - Yi, Y.J. - Sutovky, M. - Sutovsky, P.
Inhibition of proteasomal proteolysis affects expression of extracellular matrix components and steroidogenesis in porcine oocyte-cumulus complexes.
Domestic Animal Endocrinology. Roč. 42, č. 1 (2012), s. 50-62. ISSN 0739-7240. E-ISSN 1879-0054
R&D Projects: GA ČR GAP502/11/0593
Institutional research plan: CEZ:AV0Z50450515
Keywords : Oocyte-cumulus complex * TNFAIP6 * HAS2 * Progesterone * Ubiquitin * Proteasome
Subject RIV: ED - Physiology
Impact factor: 2.377, year: 2012

Porcine oocyte-cumulus complexes (OCCs) form an expanded cumulus extracellular matrix (ECM) in response to gonadotropins during meiotic maturation. Essential components of ECM are hyaluronan (HA), tumor necrosis factor a-induced protein 6 (TNFAIP6) and heavy chains (HC) of interalpha-trypsin inhibitor. To form expanded cumulus ECM, intermediate complexes (TNFAIP6-HC) must bind to HA to allow HC transfer onto HA. Protein turnover by the ubiquitin-proteasome pathway is poorly characterized in this process. It is known that the specific proteasomal inhibitor MG132 prevents cumulus expansion and formation of ECM. To determine whether inhibition of proteasomal proteolysis with MG132 affects cumulus cell steroidogenesis and expression of the cumulus expansion-related components (hyaluronan synthase type 2, HAS2, TNFAIP6) we cultured porcine OCCs and granulosa cells (GCs) in a medium supplemented with FSH/LH.
Permanent Link: http://hdl.handle.net/11104/0205064