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PIP2: A new key player in the transcription of ribosomal genes - PIP2
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SYSNO ASEP 0370738 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title PIP2: A new key player in the transcription of ribosomal genes - PIP2 Author(s) Yildirim, Sukriye (UMG-J)
Castano, Enrique (UMG-J)
Philimonenko, Vlada (UMG-J)
Dzijak, Rastislav (UMG-J) RID
Sobol, Margaryta (UMG-J) RID
Hozák, Pavel (UMG-J) RID, ORCIDSource Title Program & Abstracts, 22nd Wilhelm Bernhard Workshop. - Riga : Latvian Institute of Organic Synthesis, 2011
S. 21-21Number of pages 1 s. Action Wilhelm Berhnard Workshop /22./ Event date 22.08.2011-25.08.2011 VEvent location Riga Country LT - Lithuania Event type WRD Language eng - English Country LT - Lithuania Keywords PIP2 ; phospholipids ; nucleus ; transcription Subject RIV EB - Genetics ; Molecular Biology R&D Projects GAP305/11/2232 GA ČR - Czech Science Foundation (CSF) LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GD204/09/H084 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50520514 - UMG-J (2005-2011) Annotation Phosphatidylinositol-4,5-bisphosphate (PIP2) resides in the nucleus in a different form than the classical bilayer membrane, apparently forming specific nuclear protein complexes. There is limited data on the function of PIP2 in the nucleus. Yet, it was shown that PIP2 binding to histones reverses the inhibitory effect of histones on transcription in Drosophila (Yu et al., 1998). In this study we focused in the function of PIP2 in ribosomal gene transcription and showed that PIP2 binds to transcription machinery. Removal of PIP2 from in vitro transcription assays caused the inhibiton of transcription for ribosomal genes and it was possible to recover the transcription efficiency by adding back PIP2 to the transcription reaction. The data suggest that nucleolar PIP2 might serve as a transcription factor for ribosomal genes. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2012
Number of the records: 1