0353312 - ÚMCH 2011 RIV DK eng J - Journal Article
Dohnálek, Jan - Kovaľ, Tomáš - Lipovová, P. - Podzimek, Tomáš - Matoušek, Jaroslav
Structure analysis of group I plant nucleases.
Journal of Synchrotron Radiation. Roč. 18, Part 1 (2011), s. 29-30. ISSN 0909-0495. E-ISSN 1600-5775.
[International Symposium on Diffraction Structural Biology /3./. Paris - Orsay, 25.05.2010-28.05.2010]
R&D Projects: GA ČR GA310/09/1407; GA ČR GA521/09/1214
Institutional research plan: CEZ:AV0Z40500505; CEZ:AV0Z10100521; CEZ:AV0Z50510513
Keywords : nuclease * protein structure * crystal structure
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.726, year: 2011
DOI: https://doi.org/10.1107/S0909049510030700

Anticancer drugs attacking nucleic acids of the target cells have so far been based on animal or fungal ribonucleases. Plant nucleases have been proved to exhibit decreased cytotoxic side effects. Tomato bifunctional nuclease 1 with activity against both single-stranded and double-stranded RNA and DNA was produced in tobacco leaves as recombinant protein. The enzyme crystallizes under several different crystallization conditions. The presence of Zn2+ ions was confirmed by X-ray fluorescence. First crystallographic data were obtained.

Permanent Link: http://hdl.handle.net/11104/0192591