Isolation and characterization of the sperm surface proteins and their binding studies with zona pellucida glycoproteins

0330614 - BTÚ 2010 RIV HU eng A - Abstract
Zigo, Michal - Jonáková, Věra - Postlerová, Pavla
Isolation and characterization of the sperm surface proteins and their binding studies with zona pellucida glycoproteins.
Book of abstracts 3rd Central and Eastern European Proteomics Conference. Budapešť, 2009. s. 74-74. ISBN 978-963-9319-99-8.
[3rd Central and Eastern European Proteomics Conference. 06.10.2009-09.10.2009, Budapešť]
R&D Projects: GA MŠMT(CZ) 1M06011; GA ČR(CZ) GA303/09/1285
Institutional research plan: CEZ:AV0Z50520701
Keywords : zona pellucida * boar spermatozoa * glycoproteins * hyaluronidase
Subject RIV: CE - Biochemistry

Recognition and binding of spermatozoa to the zona pellucida (ZP) is a crucial step in the fertilization process. This is the topic of many scientific groups, and so far many molecules on the sperm surface, involved in mentioned process, were identified. Sperm surface proteins of ejaculated and capacitated boar sperms were isolated by Pierce® Cell Surface Isolation Kit and characterized by 1D, 2D electrophoreses, glycoprotein staining, hyaluronidase and proteolytic activities. Finally binding assay with ZP was performed. Results have shown the quantitative and qualitative differences in both 1D and 2D electrophoretic profiles, glycoprotein profile was similar to 1D electrophoretic one. Neither hyaluronidase nor proteolytic activities were observed. Binding assay proved that interactions occur between both ejaculated and capacitated sperms. Protein interactions of capacitated sperm with ZP glycoproteins seem to be more specific.
Permanent Link: http://hdl.handle.net/11104/0176363