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Sperm surface proteins: The origin, biochemical properties and role in reproduction
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SYSNO ASEP 0323274 Document Type C - Proceedings Paper (int. conf.) R&D Document Type The record was not marked in the RIV Title Sperm surface proteins: The origin, biochemical properties and role in reproduction Title Povrchové proteiny spermie: původ, biochemické vlastnosti a úloha v reprodukci Author(s) Jonáková, Věra (BTO-N) RID
Postlerová, Pavla (BTO-N) ORCID, RID
Davidová, Nina (BTO-N)
Tichá, M. (CZ)
Pěknicová, Jana (BTO-N) RIDSource Title Scientific session - dedicated to the 70th anniversary of the Institute of Biology and Immunology of reproduction, Scientific session. - Sofia, 2008 Pages s. 1-75 Number of pages 2 s. Action Scientific session dedicated to the 70th Anniversary of the Institute of biology and Immunology of Reproduction Event date 23.10.2008 VEvent location Sofia Country BG - Bulgaria Event type EUR Language eng - English Country BG - Bulgaria Keywords sperm surface protein ; DQH ; spermadhesin Subject RIV DN - Health Impact of the Environment Quality R&D Projects 1M06011 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA303/06/0829 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50520701 - BTO-N (2007-2013) Annotation Seminal plasma proteins bind to the sperm surface at ejaculation and may modulate sperm properties during reproduction. Porcine spermadhesins (AQN, AWN, PSP) are secreted mainly by the seminal vesicles (SV), but their mRNAs have been found also in the cauda epididymis and prostate. Using specific polyclonal antibodies, PSP-I and PSP-II proteins were immunodetected in tissue extracts from cauda epididymis, prostate, SV and Cowper´s glands on the blots, and in secretory tissues of cauda epididymis, prostate and SV by indirect immunofluorescence. We localized PSP spermadhesins on epididymal and ejaculated spermatozoa. PSP proteins are produced not only by SV and prostate, but also by epididymis. Characterization of seminal plasma proteins expressed in the individual reproductive organs might help to understand their role in the reproduction process. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2010
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